Expression of HIF-1α,TGF-β1,Mucl and MMP-9 in placenta:preliminary pathophysiological study of preeclampsia  

作　　者：吕艳关 代晓南 刘珊 魏红 高超 高莉 刘嘉茵 崔毓桂 

机构地区：[1]Laboratory of Clinical Medicine,Huai An Women & Children's Hospital [2]State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affiliated Hospital,Nanjing Medical University

出　　处：《生殖医学杂志》2012年第B12期77-86,共10页Journal of Reproductive Medicine

基　　金：supported by projects from National Foundation of Science(81170559);China 973 Program(2012CB944703)

摘　　要：Objective:To investigate expressions of hypoxia-inducible factors-la(HIF-la),transforming growth factor-β1 (TGF-pl),mucinl(Mucl) and matrix metalloproteinase-9(MMP-9) in the placenta collected from the preeclampsia patients and normal pregnant women,so as to explore the possible pathophysiological mechanism of preeclampsia. Methods:The placenta villus tissues were obtained from 35 preeclampsia women,including 16 mild preeclampsia and 19 severe preeclampsia,and 20 normal pregnant women,within 5 minutes after placental expulsion.Expressions of HIF-1α,TGF-β1,Mucl and MMP-9 were detected by Western blot.Cellular location was observed by immunohistochemistry. Results:(1) HIF-1αwas mainly located in cytoplasm and nucleus of placental villous syncytiotrophoblast. Expression level of HIF-la in the severe preeclampsia group was significantly higher than that in the mild group or control group(P<0.01).(2) TGF-pl was located in the trophoblast cell and exuviates membrane.Expression level of TGF-pl in the severe and mild preeclampsia groups was significantly higher than that in control group (P<0.05).(3) Mucl was located in trophoblast cell and exuviates membrane.Expression level of MUC1 in the severe preeclampsia group was significantly higher than that in the mild preeclampsia group and control group(P< 0.01).(4) MMP-9 was located in the trophoblast cell and villous stroma,exuviates membrane.Expression levels of MMP-9 in the two preeclampsia groups were lower than that in control group(P>0.05). Conclusion:Expressions of HIF-lα,TGF-β1 and Mucl increased in the placenta of preeclampsia group,while MMP-9 decreased.Mucl can be induced and regulated by HIF-la and TGF-β1.Over-expression of Mucl in placenta can significantly suppress the activation of MMP-9,which may influence the infiltration of trophoblast cells.The increased expression of HIF-lαand TGF-β1 in placenta may induce higher level of Mucl.This study helped us to understand the pathophysiological mechanism of preeclampsia.Objective： To investigate expressions of hypoxia-inducible factors-1α （HIF-1α）, transforming growth factor-α1 （TGF-β1）, mucinl （Mucl） and matrix metalloproteinase-9 （MMP-9） in the placenta collected from the preeclampsia patients and normal pregnant women, so as to explore the possible pathophysiological mechanism of preeclampsia. Methods： The placenta villus tissues were obtained from 35 preeclampsia women, including 16 mild preeclamp- sia and 19 severe preeclampsia, and 20 normal pregnant women, within 5 minutes after placental expulsion. Expres- sions of HIF-1α, TGF-β1, Mucl and MMP-9 were detected by Western blot. Cellular location was observed by im- munohistochemistry. Results： （1） HIF-1α was mainly located in cytoplasm and nucleus of placental villous syncytiotropho- blast. Expression level of HIF-1α in the severe preeclampsia group was significantly higher than that in the mild group or control group （P〈0.01）. （2） TGF-β1 was located in the trophoblast cell and exuviates membrane. Expres- sion level of TGF-β1 in the severe and mild preeclampsia groups was significantly higher than that in control group （P〈0.05）. （3） Mucl was located in trophoblast cell and exuviates membrane. Expression level of MUC1 in the severe preeclampsia group was significantly higher than that in the mild preeclampsia group and control group （P〈 0.01）. （4） MMP-9 was located in the trophoblast cell and villous stroma, exuviates membrane. Expression levels of MMP-9 in the two preeclampsia groups were lower than that in control group （P〉0.05）. Conclusion： Expressions of HIF-1α, TGF-β1 and Mucl increased in the placenta of preeclampsia group, while MMP-9 decreased. Mucl can be induced and regulated by HIF-1α and TGF-β1. Over-expression of Mucl in placenta can significantly suppress the activation of MMP-9, which may influence the infiltration of trophoblast cells. The increased expression of HIF-1α and TGF-β1 in placenta may induce higher level of Mucl. T

关 键 词：HIF-1 MMP-9 胎盘绒毛 病理生理 子痫 先兆 缺氧诱导因子1 滋养层细胞 

分 类 号：Q513[生物学—生物化学] Q492.6