淫羊藿有效单体对活化的肾成纤维细胞株和系膜细胞株的影响  

The Effect of Utility Monomer in Epimedium to the Renal Fibroblast Cell Line and Mesangial Cell Line

作　　者：王东[1] 张江[2] 吴同茹[1] 谢婷婷[1] 陈刚[1,3,4] 何立群[1,3,4] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]合肥师范学院,合肥230601 [3]上海市中医临床重点实验室,上海200021 [4]上海市高校中医内科E-研究院,上海200021

出　　处：《中国中西医结合肾病杂志》2012年第11期956-959,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家科技重大专项项目(No.2009ZX09311-003);国家自然基金资助项目(No.81173219);科技部中医药行业科研专项项目(No.201007005);高等学校博士学科点专项科研基金项目(No.20093107110006);上海市科委创新行动计划项目(No.11DZ1973100);上海市教育委员会E-研究院建设计划资助项目(No.E03008);上海高校创新团队建设项目(No.200908)

摘　　要：目的:观察淫羊藿有效单体对活化的肾成纤维细胞株和系膜细胞株的影响。方法:采用体外培养肾成纤维细胞株和系膜细胞株的方法,将其分为空白对照组、TGF-β12ng/ml组、TGF-β12ng/ml+中药10-5g/ml组和TGF-β12ng/ml+中药10-6g/ml组。采用MTT法观察24h中药单体对两种细胞株的作用以及ELISA法对TGF-β的分泌情况。结果:与空白对照组相比,加入TGF-β1刺激因子可以促进两种细胞株的活化(P<0.05),以及TGF-β的分泌(P<0.01),中药单体可以抑制活化的细胞株增殖和TGF-β的分泌(P<0.05或P<0.01)。结论:淫羊藿中药单体可以抑制活化的肾成纤维细胞株和系膜细胞株的增殖,有效防治肾纤维化,其作用机制可能与其抑制TGF-β的分泌有关。Objective：To observe the effect of utility monomer in Epimedium to the renal fibroblast cell line and mesangial cell line. Methods ：To culture in vitro the renal fibroblast cell line and mesangial cell line, were divided into blank control group, TGF - β1 2 ng,/ml group, TGF -β1 2 ng/ml ＋ Chinese medicine monomer 10 -5 g/ml group and TGF - β1 2 ng/ml ＋ Chinese medi- cine monomer 10 -6 g/ml group. To observe the effect of Chinese medicine monomer for 24 hours the two cell lines by MTT assay, and the secretion of TGF -β by ELISA assay. Results：Compared with blank control group, adding TGF -β1 - stimulating factor can pro- mote the two cell lines （P 〈 0.05 ）, as well as the secretion of TGF -β （P 〈 0.01 ）, Chinese medicine monomer can inhibit the acti- vation of two cell lines proliferation and secretion of TGF -β （P 〈 0.05 or P 〈 0.01 ）. Conclusion： Utility monomer of Epimedium can inhibit the activation of the renal fibroblast cell line and mesangial cell line proliferation and secretion of TGF -β, effective prevention and treatment of renal fibrosis, and its possible mechanism of action relate to inhibition of TGF - β secretion.

关 键 词：成纤维细胞株 系膜细胞株 体外培养 淫羊藿 中药单体 转化生长因子-Β 

分 类 号：R285.5[医药卫生—中药学]