马铃薯试管薯诱导影响因素分析  

Effects of Different Factors on Induction of in vitro Potato Microtubers

作　　者：金明石 严英哲 张健[2] 潘菊[2] 

机构地区：[1]朝鲜农业科学院农业生物学研究所 [2]沈阳市农业科学院,辽宁沈阳110034

出　　处：《中国马铃薯》2012年第6期332-335,共4页Chinese Potato Journal

摘　　要：以马铃薯栽培品种‘Favorita’脱毒试管苗为材料,研究了离体条件下外源激素、活性炭以及光照对试管薯形成和发育的影响。结果表明:加入一定浓度的外源激素,有利于提高‘Favorita’试管薯的产量和质量,其诱导效应依次为矮壮素(CCC)>多效唑(PP333)>CCC+6-苄氨基嘌呤(6-BA)>丁酰胺(B9)>6-BA>无激素;活性炭能够明显提高试管薯前期的形成和发育;8 h/d散射光照比全黑暗试管薯重量和大薯率都有提高;全黑暗使试管薯发生提前,结薯集中,但不利于试管薯的增重。The virus free plantlets of ＇Favorita＇ were used in this experiment. The effects of plant growth hormone and activated carbon in culture medium on the formation of in vitro stolon and tuberization were studied. The plant growth substance could improve the quantity and quality of microtuber and the inductive effects could be arranged as chlorocholine chloride （CCC） 〉 Paclobutracol （PP~） 〉 CCC ＋ 6-Benzylaminopurine （6-BA） 〉 Daminozide （Bg） 〉 6-BA 〉 no-inductive stimulus. Activated charcoal could improve tuberization and the development of microtubers at the early stage. Compared with darkness, daylength of 8 h diffused light was good for microtuber growth, and increased large sized microtuber percentage. Darkness promoted microtuber initiation and microtuber formed in a short time, but was disadvantage to increase the weight per microtuber.

关 键 词：马铃薯试管薯 诱导 外源激素 活性炭 光照 

分 类 号：S532[农业科学—作物学]