磷脂酶反应体系中丝氨酸含量的测定  

Contents determination of serine in lecithin enzyme reaction system

作　　者：朱雄伟[1] 李卫朋[1] 苏腾甲[1] 刑彦君[2] 

机构地区：[1]武汉工程大学化工与制药学院,绿色化工过程省部共建教育部重点实验室,武汉430074 [2]河南省科学院化学研究所有限公司,郑州450002

出　　处：《中国食品添加剂》2012年第6期254-257,共4页China Food Additives

摘　　要：磷脂酶是一类特殊的酯键水解酶,具有磷脂酰基转移特性,能催化含羟基化合物结合到磷脂的酰基上。在水的单相体系中可以利用磷脂酶的磷脂酰基转移特性来催化卵磷脂和丝氨酸反应,可以通过测定丝氨酸量的减少量来决定丝氨酸的转化率。本研究运用茚三酮-分光光度法检测卵磷脂和丝氨酸磷脂酶反应体系中丝氨酸的含量,测定丝氨酸最大吸收波长,并对该体系中的缓冲液pH值、茚三酮用量、反应时间进行研究。结果表明:丝氨酸最大吸收波长为570nm,缓冲液pH为6.0、茚三酮用量为1.4mL、反应时间为30min时,对丝氨酸含量的测定结果更为准确。Phospholipase is a kind of special ester hydrolase and has a unique property of transphosphatidylation, which can hydrolyze phospholipids into phosphatidic acid and the relevant hydroxy compounds. The reaction of lecithin and serine in water is catalyzed by transphosphatidylation. The conversion rate of serine is determined by the decrement of serine. Contents of serine was detected by ninhydrin - spectrophotometric and the serine maximum absorption wavelength was also determined. The pH value of buffer solutions, dosage of ninhydrin and reaction time were discussed. The result showed that the serine maximum absorption wavelength was 570 nm, determination of serine was more precisely when the pH value of buffer solution was 6. 0, dosage of ninhydrin was 1. 4 mL and the reaction time was 30 rain.

关 键 词：磷脂酶反应体系 茚三酮-分光光度法 丝氨酸 含量测定 

分 类 号：R33[医药卫生—人体生理学]