基因芯片方法快速检测MDR-TB及利福平和异烟肼耐药基因的研究  

RAPID DETECTION OF DRUG RESISTANT RELATED GENES IN MULTIDRUG-RESISTANT TUBERCULOSIS BY GENE CHIP AND RIFAMPIN AND ISONIAZID RESISTANCE GENE

作　　者：张海英[1] 高会霞[2] 许怡[2] 

机构地区：[1]河北省石家庄市第五医院皮肤科,河北石家庄050021 [2]河北省石家庄市第五医院检验科,河北石家庄050021

出　　处：《河北医科大学学报》2012年第12期1421-1424,共4页Journal of Hebei Medical University

基　　金：石家庄市科技局攻关项目(11146643)

摘　　要：目的使用基因芯片结合仪器法液体快速培养分析耐多药(multidrug resistant,MDR)结核分枝杆菌(mycobacterium tuberculosis,MTB)的耐药基因和表型特征。方法应用聚合酶链反应(polymerase chain reaction,PCR)扩增-基因芯片杂交法检测耐多药结核分枝杆菌(multidrug resistant tuberculosis,MDR-TB)菌株中利福平(rifampin,RFP)和异烟肼(isoniazid,INH)耐药基因的突变位点及类型,平行用BD MGIT960系统检测所选菌株对RFP和INH的敏感性。结果以MGIT960药敏结果作为参考标准,34株MDR-TB中,基因芯片检测MTB对RFP、INH药的符合率分别为85.29%和94.11%;32株RFP耐药突变株中22株为rpoB基因531位密码子突变,29株INH耐药突变株中24株为katG基因315位密码子突变。结论基因芯片技术可快速、有效地检出MDR-TB,可以在未获得传统细菌表型药敏结果前指导临床用药治疗。Objective To analyze the characteristics of phenotype and genotype of muhidrug resistant tuberculosis （ MDR - TB） by gene chip and liquid culture. Methods Gene chip MDR - TB kits were used for identifying the types of rifampin （RFP） and isoniazid （ INH ） antituberculosis drug resistant genes partly and BD MGIT960 was used for detecting the drug susceptibility. Results Compared with MGIT960 ,the coincidence rate of gene chip MTBDR plus was 85.29% and 94.11% in RFP -resistant strain and INH resistant strain in 34 MDR - TB strains ; Among 32 RFP - resistant mutation strains, $531L of rpoB gene accounted for 22 strains. MTB resistant to INH was caused by the mutation of katG chiefly and the S315T1 accounted for 24 strains in 29 INH - resistant strains. Conclusion Gene chip might be a rapid and effective method for the detection of MDR - TB. So, it could be used as an assistant method to guide the theranv on clinic.

关 键 词：分枝杆菌 结核 利福平 异烟肼 寡核苷酸序列分析 

分 类 号：R378.911[医药卫生—病原生物学]