龙竹和凤尾竹PpMADS1序列的扩增与序列分析  

Amplification and Sequence Analysis of PpMADS1-like Sequences in Dendrocalamus Giganteus and Bambusa Multiplex cv. Fernleaf

作　　者：黄周英[1] 桑庆亮[1] 陈怀宇[1] 孙亮先[1] 

机构地区：[1]泉州师范学院化学与生命科学学院,福建泉州362000

出　　处：《泉州师范学院学报》2012年第6期25-31,共7页Journal of Quanzhou Normal University

基　　金：福建省科技厅青年人才项目(2007F3087);泉州市科技局计划项目(2008Z15)

摘　　要：以龙竹和凤尾竹基因组DNA为模板,通过试验不同的退火温度、循环数、Mg2+浓度及DMSO浓度,获得了16条龙竹和凤尾竹PpMADS1相似序列片段,并得出稳定扩增PpMADS1相似序列的条件.对获得的龙竹和凤尾竹PpMADS1相似序列进行序列分析,发现龙竹和凤尾竹PpMADS1相似序列可根据序列长度差异分成组I和组II,并对其推出的氨基酸序列进行分析,找到了保守的paleoAP1基序和KIII区保守的氨基酸残基,并据此认为龙竹和凤尾竹PpMADS1相似序列是AP1/SQUA亚家族成员.In this paper, annealing temperature, number of cycles, concentration of Mg2＋, and concentration of DMSO are investigated to find a steady procedure for PpMADSI-like sequence amplification through Polymerase Chain Reaction （PCR） and finally sixteen PpMADSI-like sequences are obtained using genomic DNA as template DNA in Dendrocalamus giganteus and Bambusa multiplex cv. fernlea{. All sequences obtained are sorted into two groups~ group I and group II, based on sequence length polymorphism and the results of sequence analysis of putative amino acid sequences. Conserved paleoAP1 motif and conserved amino acid residues of KIII region of K box are also observed, according to which all PpMADSI-like sequences are believed to be members of AP1/SQUA subfamily of MIKC-type MADS-box gene family.

关 键 词：龙竹 凤尾竹 PpMADS1相似序列 序列分析 

分 类 号：Q523[生物学—生物化学] S718.46[农业科学—林学]