2型糖尿病肾阴虚证大鼠模型的建立及其稳定性评价  

Establish the rat model of type 2 diabetic kidney yin deficiency syndrome and evaluate its stability

作　　者：戴冰[1] 杨赛男[2] 肖子曾[2] 何泽云[1] 周芳亮[2] 黄开颜[1] 刘红宇[1] 邢瑞[2] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007

出　　处：《湖南中医药大学学报》2012年第12期5-7,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科学技术厅科技计划一般项目(2010FJ4091);长沙市科技局项目(K1106040-31);湖南省高校创新平台开放基金项目(11K046)

摘　　要：目的建立2型糖尿病肾阴虚证大鼠模型及其稳定性评价方法。方法采用灌胃高脂乳结合腹腔注射小剂量链脲佐菌素(STZ)诱导2型糖尿病大鼠模型,再灌胃糖皮质激素氢化可的松建立2型糖尿病肾阴虚证大鼠模型,继续高脂乳喂养1个月,检测大鼠模型血液中的血糖值、cAMP水平,考察该模型的稳定性。结果成功建立了高脂乳结合小剂量STZ加氢化可的松诱导的2型糖尿病肾阴虚证大鼠模型,模型组大鼠空腹血糖值≥16.7 mmoL/L,cAMP水平为(1.32±0.04)mmoL/L,且其日常表现也与中医肾阴虚证相吻合。1个月后,模型组大鼠空腹血糖值≥16.7 mmoL/L,cAMP水平为(1.24±0.02)mmol/L。结论通过灌胃高脂乳结合腹腔注射小剂量链脲佐菌素(STZ)再灌服氢化可的松成功复制出了稳定性良好的2型糖尿病肾阴虚证大鼠模型。Objective To establish a method to create a rat model of type 2 diabetic kidney yin deficiency syndrome and evaluate its stability. Methods Used the method of gavage high-fat milk and intraperitoneal injection of small doses of streptozotocin to induce the Type 2 diabetic rat model, then gavage the Hydrocortisone of Glucocorticoid establish the rat model of type 2 diabetic kidney yin deficiency syndrome, And then continue to feed a month with high-fat milk, detect the Blood glucose and cAMP levels of the rat model, study the model＇ s stability. Results Successfully established the rat model of type 2 diabetic kidney yin deficiency syndrome with high-fat milk, small doses of streptozotocin and hydrocortisone. The model group＇ s Blood glucose≥ 16.7mmol/L,the value of cAMP is 1.32± 0.04 mmol/L, and the daily characterization of the rat is similar to the kidney yin deficiency syndrome of the Traditional Chinese Medicine. The model group＇ s Blood glucose≥ 16.7mmol/L and the value of cAMP is 1.24±0.02 mmoL/L in a month later. Conclusion Successfully copy out a good stability rat model of type 2 diabetic kidney yin deficiency syndrome by gavage high-fat milk and Intraperitoneal injection of small doses of streptozotocin and then gavage the Hydrocortisone.

关 键 词：2型糖尿病 肾阴虚 大鼠 动物模型 评价 

分 类 号：R255.4[医药卫生—中医内科学]