还少丹对D-半乳糖致衰小鼠的抗衰老作用  

Research for anti-aging function of huanshaodan in aged mice induced by D-galactose

作　　者：程莉娟[1] 刘群良[1] 谭峰[1] 张波[1] 陈伶利[1] 

机构地区：[1]湖南中医药大学医学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2012年第12期11-12,14,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科技计划一般资助项目(2012FJ4096)

摘　　要：目的探讨还少丹对D-半乳糖(Dgalactose,D-gal)诱导的衰老模型小鼠的抗衰老作用。方法采用D-gal建立小鼠衰老模型,用还少丹水煎剂灌胃6周;分光光度法检测小鼠脑、肝脏组织谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxidase dismutase,SOD)活性;观察小鼠肝脏、脾脏指数的改变。结果衰老模型组小鼠肝脏指数、脾脏指数以及脑和肝脏组织的SOD、GSH-Px活性均明显降低。还少丹能显著提高衰老模型小鼠的肝脏指数和脾脏指数;显著升高脑和肝组织的SOD、GSH-Px活性。结论还少丹的抗衰老作用与其影响器官指数、升高GSH-Px和SOD活性有关,其作用机制可能通过清除体内自由基,提高免疫功能以实现延缓衰老的进程。Objective To investigate the effect and mechanism of huanshaodan on anti-aging in aged mice induced by D-galactose（D-gal）. Methods The aged model was established by injecting D-gal. After feeding with huanshaodan for 6 weeks, spectrophotometry was used to detect the activity of glutathione peroxidase and superoxidase dismutase in the brain and liver of mice. Meanwhile the liver index and spleen index of mice were also detected. Results The liver index, spleen index, and the activity of glutathione peroxidase and superoxidase dismutase of brain and liver in the aged model group were significantly reduced, whereas the liver index, spleen index,the activity of superoxidase dismutase and glutathione peroxidase in the brain and liver were elevated remarkably. Conclusion The activity of glutathione peroxidase and superoxidase dismutase in the brain and liver of aged mice can be elevated by huanshaodan, and so do the liver index and spleen index, which suggest that huanshaodan has an anti-aging effect through eliminating free radicals and improving the immunity.

关 键 词：还少丹 D-半乳糖诱导致衰模型 抗氧化 

分 类 号：R285.5[医药卫生—中药学]