黄连配伍降低马兜铃酸A含量的研究  

Study on Rhizoma Coptidis Decreasing the Content of Aristolochic Acid A by HPLC

作　　者：吴建红[1] 张智华[1] 吕银娟[1] 孙康[2] 李汉鑫[3] 

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430065 [2]湖北中医药大学 [3]湖北中医药大学期刊社,湖北武汉430065

出　　处：《湖北中医药大学学报》2012年第6期39-42,共4页Journal of Hubei University of Chinese Medicine

基　　金：湖北中医药大学院课题(中医科(2005)11号;中医科(2010)22号)

摘　　要：目的通过检测关木通、广防己不同药物配伍组中马兜铃酸A的含量,探寻降低肾毒性的最佳配伍组。方法 (1)色谱柱为symmetry ODS(C18,4.6mm×250mm,5μm);流速:1mL/min;温度:室温;检测波长:250nm;流动相:乙腈-(水+冰醋酸)为58∶42(v/v)。(2)色谱柱为Diamon C18(250mm×4.6mm 5μm);柱温:30℃;流速:1.0mL/min;检测波长:316nm;流动相:乙腈-(醋酸-三乙胺)为45∶55(v/v)。结果 (1)与关木通组比较,木通茯苓组、导赤散+当归组、导赤散组、木通栀子组、木通黄连组和导赤散+黄连组的马兜铃酸A的含量分别为:0.1706、0.1402、0.1219、0.1195、0.0778、0.0442和0.0265mg/L。(2)与广防己组比较,广防己生晒参组、广防己肉桂组、广防己茯苓组、广防己生晒参肉桂茯苓组、广防己黄连组和广防己生晒参肉桂茯苓黄连组的马兜铃酸A的含量分别为:1.6830、0.4960、0.3930、0.3700、0.2930、0.1760和0.0520mg/L。结论配伍黄连后,马兜铃酸A的含量能显著降低,黄连与关木通、黄连与广防己是减毒的最适配伍。Objective To research into the optimal compatibility of decreasing nephrotoxic substance,with high-performance liquid chromatography（HPLC） method for determination of the content of aristolochic acid A in Caulis aristolochiae manshuriensis,Aristolochia fangchi after Rhizoma coptidis was matched.Methods（1）The analytical column was symmetry ODS C18 column（4.6mm×250mm,5μm）,the mobile phase consisted of a mixture,acetonitrile-acetic acid 58∶ 42（v/v）.The detection wavelength was 250nm.The column was at temperature room.The flow rate was 1.0mL/min.（2）Diamon C18 column（250mm×4.6mm,5μm）used as chromatographic column,acetonitrile-（acetic acid-triethylamine）45∶ 55（v/v）as mobile phase,a parameter 316nm as the detection wavelength,30 degrees centigrade selected as the column temperature and a 1.0mL/min as the velocity.Results（1）The contents of aristolochic acid A in Gmt group,GmtF group,DcsD group,Dcs group,GmtZ group,GmtH group,DcsH group were 0.1706、0.1402、0.1219、0.1195、0.0778、0.0442 and 0.0265mg/L individually.（2）The contents of aristolochic acid A in Gfj group,GfjS group,GfjR group,GfjF group,GfjSRF group,GfjH group,GfjSRFH group were 1.6830、0.4960、0.3930、0.3700、0.2930、0.1760 and 0.0520mg/L individually.Conclusion The concentration of aristolochic acid A was decreased in Caulis aristolochiae manshuriensis,Aristolochia fangchi after Rhizoma coptidis was matched.It was optimal compatibility of decreasing nephrotoxic substance that Rhizoma coptidis matched Caulis aristolochiae manshuriensis or Aristolochia fangchi.

关 键 词：黄连 关木通 广防己 配伍 高效液相色谱法 马兜铃酸A 

分 类 号：R284.2[医药卫生—中药学]