HPLC法同时测定秦艽中龙胆苦苷和牛膝中蜕皮甾酮的含量  

Determination of gentiopicrin and ecdysterone in Gentiana macrophylla Pall and Achyranthes bidentata Bl by HPLC

作　　者：阮洪生[1,2] 苗晶囡[1] 马丁[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040 [2]黑龙江八一农垦大学生命学院

出　　处：《黑龙江八一农垦大学学报》2012年第6期33-35,40,共4页journal of heilongjiang bayi agricultural university

摘　　要：建立同时测定秦艽中龙胆苦苷和牛膝中蜕皮甾酮含量的RP-HPLC分析方法。采用Kromacil C18色谱柱(4.6 mm×250 mm,5μm);流动相:乙腈(A)-0.1%醋酸水溶液(B)进行梯度洗脱;流速1.0 mL·min-1;检测波长:254 nm;柱温:25℃。龙胆苦苷、蜕皮甾酮的进样浓度分别在0.80～20.00,0.40～10.00μg.mL-1范围内线性关系良好;平均回收率(n=6)分别为100.02%,99.95%。结论:该方法简便,准确,重现性好,具有较强的应用价值。To develop an HPLC method for determining the content of gentiopicrin and ecdysterone in Gentiana macrophyUa Pall and Achyranthes bidentata B1.A Kromacil Cls column （4.6mm×250mm,5μm） was adopted,the mobile phase was acetonitrile-water （contained 0.1% acetic acid） for gradient elution with flow rate of 1.0 mL·min^-1.The detection was set at 254 nm,and the column temperature was maintained at 25 ℃.Good linearities were obtained from 0.80 to 20.00,0.40 to 10.00 μg·mL^-1 for gentiopicrin and ecdysterone,respectively. The mean recoveries （n=6） of 2 components were 100.02%,99.95%,respectively. Conclusion： The method is simple and accurate with good reproducibility.

关 键 词：HPLC 秦艽 龙胆苦苷 牛膝 蜕皮甾酮 

分 类 号：R283.6[医药卫生—中药学]