丹尼斯凤梨细菌性软腐病拮抗细菌的筛选及鉴定  

Screening and identification of antagonistic bacteria against bacterial soft rot of Guzmania denise

作　　者：王寅寒[1] 谭志琼[1] 刘海[1] 张荣意[1] 

机构地区：[1]海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院,海南海口570228

出　　处：《广东农业科学》2012年第20期63-64,67,共3页Guangdong Agricultural Sciences

基　　金：海南省热带生物资源可持续利用重点实验室开放基金(2011年);海南大学"211工程"建设经费资助项目(2010年)

摘　　要：从丹尼斯凤梨根际土壤中筛选到687株菌株,其中菌株A2对丹尼斯凤梨细菌性软腐病具有高效拮抗作用,进行形态观察,生理生化特性测定及16S rDNA序列分析,将菌株A2鉴定为枯草芽孢杆菌(Bacillus subtilis)。将菌株A2的抑菌作用与几种杀菌剂的抑菌作用相对比,发现A2的抑菌作用仅次于农用链霉素。687 bacterial strains were obtained from rhizosphere soil of Guzmania denise,in which strain A2 had a strong antagonism to bacterial soft rot of Guzmania denise.Strain A2 was identified as Bacillus subtilis based on its morphological features,physiological and biochemical characteristics and 16S rDNA gene sequence.Bacteriostatic effect of strain A2 was compared with some bacteriocides,which was second only to Streptomycin.

关 键 词：丹尼斯凤梨 细菌性软腐病 拮抗细菌 鉴定 

分 类 号：S436.679[农业科学—农业昆虫与害虫防治]