量子点荧光探针用于汉坦病毒重组抗原的检测  

Detection of recombinant Hantavirus antigens by using water-solubility quantum dots fluorescent probe

作　　者：陈琼[1,2] 郑锦峰[3] 孙军红[4] 姚苹苹[5] 王洁[1] 李晶[2] 邓小昭[2] 王长军[2] 朱函坪[5] 张云[1,2] 

机构地区：[1]南京医科大学公共卫生学院,江苏南京210029 [2]南京军区疾病预防控制中心,江苏南京210002 [3]南京军区南京总医院营养科,江苏南京210002 [4]南京军区联勤部卫生部,江苏南京210016 [5]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《东南国防医药》2012年第6期483-486,共4页Military Medical Journal of Southeast China

基　　金：国家自然科学基金(81171609);浙江省自然科学基金(Y205749)

摘　　要：目的应用量子点荧光探针对汉坦病毒(Hantavirus,HV)重组抗原进行检测。方法合成水溶性量子点荧光纳米颗粒,并在其表面修饰G蛋白和anti-HV抗体作为量子点荧光探针,对HV重组抗原进行检测并优化检测条件。结果量子点与抗体的最佳偶联条件:pH 6.0、反应时间2 h、抗体浓度为20μg/ml。用本方法检测HV重组抗原的最低检测值为5 ng/ml。结论该探针能有效的识别HV抗原,且操作简便快速,为HV重组抗原的检测和肾出血热综合征的诊断提供了新方法。Objective To detect recombinant Hantavirus antigens by using a quantum dots （QDs） fluorescent probe. Methods The probe of water-solubility QDs fluorescence nanoparticle was synthesized and modified with protein G and anti-HV Antibodies. We detected recombinant Hantavirus antigens by probe combining immune method, and optimized the detecting conditions. Results The optimum reaction time, pH and goat antibodies concentration for conjugating the QDs were 2 h,6.0, and 20 μg/ml, respectively. The limit of detection of recombinant antigens was 5 ng/ml. Conclusion The fluorescent probe could effectively recognize HV antigens. The method was specific, sensitive and convenient. We developed a new method for HV antigens detection.

关 键 词：量子点荧光探针 汉坦病毒 重组抗原 

分 类 号：R446.621[医药卫生—诊断学]