RNA二级结构在siRNA设计中的应用  

Application of RNA secondary structure in siRNA design

作　　者：桂坚斌[1] 孙迎[1] 高武[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《北京生物医学工程》2012年第6期652-656,共5页Beijing Biomedical Engineering

摘　　要：RNA干扰(RNA interference,RNAi)是一种基因沉默现象,通过短双链RNA(smal linterfering RNA,siRNA)降解靶基因的信使RNA(message RNA,mRNA)以抑制基因的表达。在RNAi的应用中,设计高效的siRNA对RNAi的成功有重要作用。关于siRNA的设计已有很多设计原则可供参考,如MPI准则、Tuschl法则等。在此基础上,亦有研究进一步考察了RNA二级结构对siRNA沉默效率的影响,并建立相关模型进行分析,如氢键系数、排斥环模型等。本文就RNA二级结构在siRNA设计过程中模型的建立及结论的验证做出论述与分析。RNA interference （RNAi） is a kind of gene silencing. Small interfering RNA （siRNA） is used to suppress gene expression by degrading the message RNA （mRNA） of the target gene. Effective siRNAs are important in RNAi experiments. There are a lot of principles for siRNA design, such as MPI basic principles and Tuschl principles and so on. On the basis of these studies, some have made further research on the relationship between RNA secondary structure and silencing efficiency of siRNA,and several models have been established,such as H-b index,spelling loops and so on. In this paper,we discuss and analyze the studies of RNA secondary structure in siRNA design.

关 键 词：RNA干扰 二级结构 SIRNA 沉默效率 

分 类 号：R318.04[医药卫生—生物医学工程]