RP-HPLC法同时测定补肾益脑胶囊中五味子醇甲、五味子甲素和五味子乙素的含量  

Content determination of Schisandrin、Deoxyschizandrin and γ-schizandrin in Bushenyinao Capsule simultaneouly by RP-HPLC

作　　者：陈恩瑜 施夏蓉 洪可俊 

机构地区：[1]福建省漳州市药品检验所,漳州363000

出　　处：《海峡药学》2012年第12期49-52,共4页Strait Pharmaceutical Journal

摘　　要：目的建立用RP-HPLC(反相高效液相色谱)法同时测定补肾益脑胶囊中五味子醇甲、五味子甲素和五味子乙素含量的方法。方法色谱柱为Agilent C18,流动相为甲醇-水(70∶30),检测波长为250nm,流速为0.8mL·min-1,进样量为10μL。结果五味子醇甲、五味子甲素和五味子乙素进样浓度的线性范围分别为10.17～203.37μg·mL-1(r=0.9997)、2.17～43.43μg·mL-1(r=0.9997)、2.82～56.40μg·mL-1(r=0.9995),平均回收率分别为97.9%(RSD=1.45%,n=9)、98.7%(RSD=1.71%,n=9)、97.4%(RSD=1.56%,n=9)。结论本方法结果准确,重复性好,可用于补肾益脑胶囊的质量控制。OBJECTIVE To establish a RP-HPLC method for the content determination of Schisandrin、Deoxyschizandrin and γ-schizandrin in Bushenyinao Capsule.METHODS The separation was performed on Agilent C18 column with mobile phase of methanol-water（70∶30） at detection wavelength of 250nm,the flow rate was set at 0.8mL·min-1 and the injection volumn was 10μL.RESULTS The linear range were 10.17～203.37μg·mL-1（r=0.9997）for schisandrin with an average recovery of 97.9%（RSD=1.45%,n=9） and 2.17～43.43μg·mL-1（r=0.9997） for deoxyschizandrin with an average recovery of 98.7%（RSD=1.71%,n=9） and 2.82～56.40μg·mL-1（r=0.9995） for γ-schizandrin with an average recovery of 97.4%（RSD=1.56%,n=9） CONCLUSION The method is accurate and reproducible for quality control of Bushenyinao Capsule.

关 键 词：RP-HPLC 补肾益脑胶囊 五味子醇甲 五味子甲素 五味子乙素 含量测定 

分 类 号：R927.2[医药卫生—药学]