泡沫分离法提取竹节参总皂苷的工艺优选  

Optimization of Extraction Technology for Total Saponins from Panax japonicus by Foam Separation

作　　者：张海滨[1] 何毓敏[1] 张长城[1] 关乔中[2] 毛帅[2] 孙志伟[1] 袁丁[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《中国实验方剂学杂志》2013年第1期18-20,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31070314)

摘　　要：目的:优选泡沫分离法提取竹节参总皂苷的工艺条件。方法:以富集比、纯度比、回收率为指标,对气泡大小、原料液温度、电解质质量浓度及pH等影响泡沫分离的主要因素进行考察,采用单因素试验优化泡沫分离法提取竹节参皂苷的工艺条件。结果:最佳提取工艺为气泡直径0.4～0.5 mm,温度65℃,pH 5.5,电解质NaCl摩尔浓度0.015 mol·L-1,总皂苷富集比2.1,纯度比2.6,回收率98.33%。结论:泡沫分离法分离竹节参总皂苷方法稳定可靠,产品收率高,为天然竹节参皂苷的开发利用提供实验依据。Objective： To optimize extraction conditions of total saponins from Panax japorticus by foam separation technique. Method： Taking enrichment ratio, purity ratio and recovery as indexes, effect of pH, bubble size, temperature of mixture, electrolyte concentration and other main influencing factor on foam separation was investigated, single factor test was used to optimize extraction technology of total saponins from P. japonicus by foam separation. Result： Optimum extraction conditions were as followings： bubble diameter 0.4-0.5 mm, temperature 65 ℃ , pH 5.6, the concentration of NaCl0. 015 mol·L^-1, under these conditions, enrichment ratio of total saponins was 2. 1, purity ratio was above 2.6 and recovery was 98.33%. Conclusion： Foam separation technique could be applied to separate total saponins from P. japonicus with stability, reliability and high yield, it provided a reference for development and utilization of natural total saponins in P. japonicus.

关 键 词：泡沫分离 竹节参皂苷 富集比 回收率 纯度比 

分 类 号：R283.6[医药卫生—中药学]