正交试验法优选云南松松塔总黄酮的提取工艺  

Optimization of Extraction Technology of Total Flavonoids from Pinecone of Pinus yunnanensis by Orthogonal Test

作　　者：李冬梅[1] 李莉[1] 吴五谊[1] 刀志琪[1] 刘光明[1] 

机构地区：[1]大理学院药化学院,云南大理671000

出　　处：《中国实验方剂学杂志》2013年第1期37-39,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860365)

摘　　要：目的:优选云南松松塔总黄酮的提取工艺。方法:采用超声波辅助法提取云南松松塔总黄酮,以总黄酮提取率为指标,考察料液比、提取温度、乙醇体积分数和提取时间对总黄酮提取率的影响,通过正交试验法优选总黄酮的提取工艺。结果:最佳提取工艺为加30倍量60%乙醇于60℃超声辅助提取30 min,云南松松塔总黄酮质量分数3.23%。结论:优选的提取工艺省时、稳定、提取率高。Objective： To optimize extraction technology of total flavonoids in pinecone of Pinus yunrtanensis. Method： Total flavonoids in pinecone of P. yunnanensis were extracted by ultrasonic assisted extraction method, with extraction rate of total flavonoids as index, orthogonal test was applied to investigate influence of four factors （including ratio of material-liquid, ultrasonic extraction temperature, ethanol concentration and extraction time） on extraction technology of total flavonoids from pinecone of P. yunnanensis. Result： Optimum extraction technology was as following： ultrasonic assisted extracted 30 min with 30 times the amount of 60% ethanol at 60 ℃ , the content of total flavonoids in pinecone of P. yunnanensis was 3.23%. Conclusion： Optimized extraction technology was simple and stable with high extraction rate of total flavonoids.

关 键 词：云南松 松塔 总黄酮 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]