HPLC同时测定裸花紫珠药材中毛蕊花糖苷和木犀草素  

Determination of Acteoside and Luteolin in Callicarpa nudiflora by HPLC

作　　者：蔡金平[1] 董琳[2] 盛琳[2] 刘明生[1,3] 杨艳[1] 胡波[1] 叶小玲[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]海南医学院,海口571101 [3]海南省南药黎药研究院,海口571101

出　　处：《中国实验方剂学杂志》2013年第1期81-84,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI01B07);海南省重点科技计划项目(ZDXM20100040);2010年度海南省自然科学基金项目(310048)

摘　　要：目的:建立同时测定裸花紫珠药材中毛蕊花糖苷和木犀草素的方法。方法:采用高效液相色谱法,PhenomenexGemini C18(4.6 mm×250 mm,5μm)柱,以甲醇(A)-0.3%磷酸水溶液(B)为流动相线性梯度洗脱,流速1.0 mL·min-1,检测波长350 nm,柱温30℃。结果:毛蕊花糖苷和木犀草素线性范围分别在29.6～740.0,1.0～25.0μg(r=0.999 9),平均回收率分别为99.08%,99.93%,RSD分别为1.53%,1.65%。结论:方法精密度高,重复性好,可用于裸花紫珠药材中毛蕊花糖苷和木犀草素的含量测定。Objective： To establish an HPLC method for determination of acteoside and luteolin in Callicarpa nudiflora. Method： HPLC method was applied on Phenomenex Gemini Cls column （4.6 mm × 250 mm, 5 μm） at 30 ℃ under the condition of gradient elution with mobile A （methanol） and mobile B （0.3% phosphate）. The flow rate was 1.0 mL-min^-1, the detection wavelength was set at 350 nm. Result： Two components had a good liner relationship in concentration of 29.6-740.0, 1.0-25.0 μg （r = 0. 999 9）. The average recoveries were 99.08% , 99.93% and the RSD was 1.53% , 1.65% respectively. Conclusion： The method is accurate and reproducible for determination of acteoside and luteolin in C. nudiflora.

关 键 词：裸花紫珠 毛蕊花糖苷 木犀草素 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]