HPLC-ELSD同时测定链荚豆中胡萝卜苷和β-谷甾醇含量  

Simultaneous Determination of Daucosterol and β-sitosterol in Alysicarpus vaginalis by HPLC-ELSD

作　　者：李慧宁[1] 刘洪蛟[1] 付小帅[1] 姜珍[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国实验方剂学杂志》2013年第1期119-121,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定链荚豆中胡萝卜苷和β-谷甾醇含量的方法。方法:采用高效液相色谱-蒸发光散射检测法,色谱柱为Diamonsil C18柱(4.6 mm×150 mm,5μm),流动相甲醇-水(95∶5),流速1.0 mL·min-1,漂移管温度80℃,载气体积流量2.5 L·min-1。结果:胡萝卜苷和β-谷甾醇浓度分别在10～100 mg·L-1(r=0.999 3)和25～250 mg·L-1(r=0.999 1)线性关系良好;平均加样回收率分别为99.5%,98.9%,RSD分别为2.4%,2.1%。结论:该法专属、简便、准确,可用于同时测定链荚豆中胡萝卜苷和β-谷甾醇,为链荚豆的质量控制提供依据。Objective： To develop a new method for simultaneous determination of daucosterol and β-sitosterol in Alysicarpus vaginalis. Method： HPLC-ELSD method was used to determine daucosterol and β-sitosterol. The separation was performed on a Diamonsil Cla column （4.6 mm x 150 mm, 5 Ixm） with methanol- water （95： 5） as the mobile phase at the flow rate of 1.0 mL .min^-1. The temperature in drift tube was 80 ℃ and the carrier gas flow was 2.5 L .min^-1. Result： The linear ranges of daucosterol and β-sitosterol were 10-100 μg . mL^-1 （r =0. 999 3） and 25-250 mg .L^-1 （r = 0. 999 1） , respectively. The average recoveries were 99.5% , 98.9% , and RSDs were 2.4% and 2. 1% , respectively. Conclusion： This method is selective, simple, accurate, which can be applied for simultaneous determination of daucosterol and β-sitosterol in A. vaginalis.

关 键 词：链荚豆 胡萝卜苷 Β-谷甾醇 高效液相色谱-蒸发光散射检测法 

分 类 号：R284.1[医药卫生—中药学]