甘宝胶囊对小鼠急性肝损伤的保护作用  

Protective Effects of Ganbao Capsule on Liver Injury in Mice

作　　者：曹国琼[1] 张永萍[1] 隋艳华[1] 肖承鸿[1] 

机构地区：[1]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2013年第1期264-267,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化项目(黔科合中药字[2011]5032号)

摘　　要：目的:研究甘宝胶囊对四氯化碳(CCl4)、乙醇、扑热息痛所致小鼠急性肝损伤的保护作用。方法:取昆明种小鼠60只,随机分为6组:空白对照组,肝损伤模型对照组,甘宝胶囊0.125,0.25,0.5 g·kg-1剂量组,联苯双酯阳性对照组0.2 g·kg-1;ig给药,每日1次,至设定日期末分别用CCl4、乙醇、扑热息痛造成急性肝损伤模型。比色法检测血清中的丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和碱性磷酸酶(AKP)的水平,同时测定肝脏系数,此外CCl4造模组测溶血素、脾脏和胸腺系数。结果:甘宝胶囊高、中剂量组能明显降低由CCl4、乙醇、扑热息痛致肝损伤模型的ALT,AST,肝脏系数(P<0.05或P<0.01)。高剂量组能显著降低扑热息痛造模小鼠AKP的活性(P<0.05),并显著升高CCl4造模小鼠的溶血素含量,降低胸腺系数及脾脏系数(P<0.05或P<0.01)。结论:甘宝胶囊对四氯化碳、乙醇、扑热息痛致肝损伤具有明显的保护作用。Objective： To study, the protect effects of Ganbao capsule on tetrachlotide （CCl4） ethanol and acetaminophen in mice. Method： Sixty KM mice randomly： blank control group, model group with 0. 25, 0. 5 g .kg^-1 respectively） , positive control acetaminophen, were accordingly given to mice by liver group er-injury induced by carbon were divided into 6 groups injury, three experimental groups （Ganbao capsule 0. 125, （ biphenyl oral gavage once double ester 0. 2 g.kg ^-1 ）. CCl4, ethanol and in order to make the acute liver injury model at the end of experiment. Serum alanine aminotransferase phosphatase （AKP） were measured, the liver index was （ ALT ） aspartate aminotransferase （AST） and alkaline simultaneously determined. In addition in CCl4-induced model, hemolysin, spleen and thymus indexes were measured. Result： Ganbao capsule high, middle dose could obviously reduce ALT, AST, liver index for the liver injury models （ P 〈 0.05 or P 〈 0. 01 ）. High dose could reduce AKP＇s content in the mice model induced by acetaminophen （P 〈 0.05 ）, and significantly increase hemolysin content in the CCl4 model, and reduce the thymus index and spleen index in the same model （P 〈 0.05 or P 〈0. 01 ）. Conclusion： Ganbao capsule has the function of protecting the liver from liver injury induced by CCl4, ethanol and acetaminophen.

关 键 词：甘宝胶囊 肝损伤 四氯化碳 扑热息痛 

分 类 号：R285.5[医药卫生—中药学]