水稻纹枯病菌pTHPR1转化子的生物学特性  

Biological characteristics of pTHPR1 transformants of rice sheath blight fungus Rhizoctonia solani Kühn

作　　者：杨迎青[1,2] 李明海[1,3] 杨媚[1] 郑丽[1] 赵东[1] 周而勋[1] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]江西省农业科学院植物保护研究所,南昌330200 [3]华侨大学学科建设办公室,泉州362011

出　　处：《华中农业大学学报》2013年第1期34-39,共6页Journal of Huazhong Agricultural University

基　　金：国家公益性行业(农业)科研专项(nyhyzx3-16)

摘　　要：构建了根癌农杆菌介导的水稻纹枯病菌遗传转化系统,并获得了一批pTHPR1转化子。为明确这些转化子的生物学特性,在转化子库中抽取10个经过验证的转化子,从温度、pH值、碳源、氮源和光照5个方面比较了它们与野生型菌株(GD-118)在生长速度、菌落颜色、菌核颜色和菌核数量的差异。结果表明:在25℃和35℃且pH值为5.0、7.0、8.0时,以葡萄糖和半乳糖为碳源、以甘氨酸为氮源且完全黑暗的条件下培养,转化子与野生型菌株的菌丝生长速度有明显差异,有些转化子菌丝生长速度明显快于野生型菌株,有些转化子菌丝生长速度则明显慢于野生型菌株;在10℃和35℃且pH值为4.0、5.0、6.0时,以葡萄糖为碳源、以硝酸钠和硝酸钾为氮源且完全黑暗的条件下培养,转化子与野生型菌株的菌核数量和颜色均有明显差异。In the previous study, the Agrobacteriurn tumefaciens-mediated transformation system for rice sheath blight fungus Rhizoctonia solani Ktihn was established and a lot of pTHPR1 transforma- nts was obtained in our laboratory. To confirm the biological characteristics of pTHPR1 transformants, 10 verified transformants were selected from the pTHPR1 transformant library. The differences in bio- logical characteristics such as growth rate, colony colour, selerotial colour, sclerotial number and other aspects between the 10 transformants and the wild type isolate （GD-118） were compared in 5 aspects, i.e. temperature, pH value, carbon sources, nitrogen sources and illumination. The results revealed that the growth rates of these transformants had greater differences when compared with that of the wild type isolate at the following conditions, i.e. cultivation temperature at 25 ℃ and 35℃, pH 5.0, pH 7.0 and pH 8.0 of PDA at cultivation phase, glucose and galactose as the carbon sources, glyein as the nitrogen sources, and full dark as the illumination condition. The growth rates of several transformants were faster and the others were slower than that of the wild type isolate. The results also indicated that the sclerotia of transformants had greater differences in both numbers and colours when compared with that of the wild type isolate at the following conditions, i.e. cultivation temperature at 10 ℃ and 35 ℃, pH 4. 0, pH 5.0 and pH 6.0 of PDA at cultivation phase, glucose as the carbon sources, sodium nitrate and potassium nitrate as the nitrogen sources, and full dark as the illumination condition.

关 键 词：水稻纹枯病 立枯丝核菌 pTHPR1转化子 菌丝 菌核 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]