匙吻鲟的消化酶分布及其活性  

Activity and distribution of digestive enzymes for paddlefish(Polyodon spathula)

作　　者：黄瑾[1] 熊邦喜[1] 陈洁[1] 王琴[1] 朱玉婷[1] 施培松[1] 徐薇[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2013年第1期110-115,共6页Journal of Huazhong Agricultural University

基　　金：国家"973"计划(2009CB118706);国家"十一五"支撑计划项目(2006BAD03B02-04)

摘　　要：对池塘养殖条件下的匙吻鲟消化器官中胃蛋白酶、胰蛋白酶、脂肪酶和淀粉酶活性进行研究,并分析该4种酶活性在试验期间随时间的变化规律。结果表明,胃蛋白酶在食道中活性最高,其次为肝脏、胃、幽门盲囊,肠道最低;胰蛋白酶和脂肪酶活性都表现为十二指肠高于肝脏、幽门盲囊,食道居中,胃最低,且在匙吻鲟胃中脂肪酶活性极低;淀粉酶活性也是在十二指肠中最高,其次为食道、胃和肝脏,幽门盲囊最低。在6-10月的试验期间,消化器官胃蛋白酶活性呈现先降低后升高的变化过程,8月份最低;胰蛋白酶活性随试验时间的延长不断升高;脂肪酶和淀粉酶活性则表现出先升高后降低的变化过程,9月份达到最高值。The enzyme activities, distribution features and changing patterns with feeding time of pepsin, trypsin, lipase and amylase in digestive organs for pond-cultured paddlefish were determined in the present study. The results showed that the highest activity of pepsin was found in the oesophagus, then followed in order by the liver, stomach, pyloric caeca, and intestine tract. The duodenum had the highest activity of trypsin and lipase, then followed in order by the liver, pyloric caeca, stomach, and oe- sophagus. The activities of amylase from the highest to the lowest in the digestive organs was in the or- der of the duodenum, oesophagus, stomach, liver, and pyloric caeca. During the culturing period from June to October,the activity of pepsin decreased at first, and then increased, with the lowest value ap- peared in August. The activities of trypsin increased gradually with increasing of the feeding time. While the activities of lipase and amylase increased at first and then declined, with the highest value appeared in September.

关 键 词：匙吻鲟 消化器官 消化酶 池塘 鱼类养殖 

分 类 号：Q959.463[生物学—动物学]