激动肝郁脾虚证大鼠海马CA1区AMPA受体对杏仁核区AMPA受体亚基基因表达的影响及逍遥散的调节作用  

Influence of Xiaoyao Powder on gene expression of GluR1 mRNA, GluR2 mRNA in amygdala and hippocampus in rat model of liver-stagnation and spleen-deficiency syndrome

作　　者：岳利峰[1] 王玲[2] 王福[1] 奚胜艳[3] 陈昌乐[1] 赵健[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京航空航天大学,北京100191 [3]厦门大学,厦门361005

出　　处：《中华中医药杂志》2013年第1期64-67,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学青年基金(No.61101008);北京中医药大学自主选题项目(No.2009JYBZZ-JS006)~~

摘　　要：目的:探讨逍遥散治疗肝郁脾虚证的基因调节机制。方法:25只SD雄性大鼠随机等分为5组,正常组、模型组、假手术组、α-氨基羟甲基恶唑丙酸(AMPA)组和逍遥散组。以21d慢性束缚应激方法造肝郁脾虚证模型组。假手术组、AMPA组和逍遥散组均采用21d慢性束缚联合脑部埋管微量注射方法造模,AMPA组在双侧海马CA1区埋管微量注射AMPA,逍遥散组灌服逍遥散溶液。运用RT-PCR一步法检测海马CA1区和杏仁核区AMPA受体的重要亚基GluR1 mRNA和GluR2 mRNA的表达变化。结果:在杏仁核区,与AMPA组比较,逍遥散组GluR1mRNA和GluR2 mRNA表达差异无统计学意义;在海马CA1区,与正常组比较,AMPA组GluR1 mRNA和GluR2mRNA表达差异均有统计学意义(P<0.01,P<0.05);与AMPA组比较,逍遥散组GluR1 mRNA和GluR2 mRNA表达差异有统计学意义(P<0.01)。结论:结合前期实验,从基因表达角度,进一步推断逍遥散通过纠正杏仁核和海马AMPA受体的"兴奋-抑制"失衡,重建稳态,来治疗肝郁脾虚证。Objective：To further investigate the mechanism of gene regulation of Xiaoyao Powder in the treatment of liver-stagnation and spleen-deficiency syndrome.Methods：All SD rats（n=25） were randomly divided into 5 equal groups,including the normal group,model group,sham-operation group,AMPA group and Xiaoyao Powder group.The model was established by chronic restraint for 21 days.The last 3 groups were modeled by chronic restraint for 21 days in combination with micro-injection by cerebral stereotaxis instrument.AMPA group was micro-injection with AMPA.Xiaoyao Powder group was fed with solution of Xiaoyao Powder.Results： In amygdale,compared with AMPA group,the expressions of GluR1 mRNA and GluR2 mRNA had no statistical significance in Xiaoyao Powder group.In CA1 region,compared with normal group,the expressions of GluR1 mRNA and GluR2 mRNA had no statistical significance in Xiaoyao Powder group,while had statistical significance（P0.01,P0.05） in AMPA group;compared with AMPA group,the expressions of GluR1 mRNA and GluR2 mRNA had statistical significance（P0.01） in Xiaoyao Powder group.Conclusion： The regulating mechanism of Xiaoyao Powder to the expressions of subunits of hippocampus and amygdala AMPA receptor may be coincidence with that of AMPA in rat model of liver-stagnation and spleen-deficiency syndrome.It is deduced that Xiaoyao Powder cures liver-stagnation and spleen-deficiency syndrome through correcting the disorder of ＇excitation-inhibition＇ of hippocampus and amygdala and reconstructing the homeostasis.

关 键 词：逍遥散 肝郁脾虚证 基因表达 调节机制 α-氨基羟甲基恶唑丙酸 α-氨基羟甲基恶唑丙酸受体 

分 类 号：R285.5[医药卫生—中药学]