葛根素对大鼠脑缺血再灌注损伤α7nAChR、NF-кBp65及STAT3 mRNA表达的影响  

Effect of puerarin on mRNA expression of α7nAChR,NF-кBp65 and STAT3 in brain after cerebral ischemia-reperfusion in rats

作　　者：梅志刚[1] 王明智[1] 刘晓洁[1] 曾永保[1] 

机构地区：[1]三峡大学医学院国家中医药管理局中药药理科研三级实验室,宜昌443002

出　　处：《中华中医药杂志》2013年第1期113-117,共5页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:从胆碱能抗炎通路探讨葛根素对脑缺血再灌注大鼠脑组织α7烟碱型乙酰胆碱受体(α7nAChR)、核转录因子-кB(NF-кBp65)及信号转导子与转录激活子3(STAT3)mRNA表达的影响。方法:60只雄性SD大鼠,随机分为5组。葛根素组、α银环蛇毒素(α-BGT)组大鼠尾静脉注射葛根素;尼古丁组尾静脉注射尼古丁,假手术组和模型组大鼠均注射等量0.9%氯化钠溶液。预给药5d后,应用线栓法建立大鼠右侧大脑中动脉闭塞模型,缺血再灌注即刻;α-BGT组尾静脉注射α-BGT。再灌注24h后,观察脑梗死面积,RT-PCR法检测脑组织α7nAChR、NF-кBp65及STAT3 mRNA的表达。结果:与模型组比较,葛根素组和尼古丁组大鼠脑梗死面积显著缩小,缺血脑组织α7nAChR、STAT3 mRNA的表达显著升高,NF-кBp65 mRNA的表达显著下降(P<0.01,P<0.05);α-BGT可阻断葛根素对上述指标的影响。结论:葛根素对脑缺血再灌注具有较好的抗炎保护作用,其机制可能与激活胆碱能抗炎通路有关。Objective： To explore the effect of puerarin on mRNA expression of alpha7 nicotinic acetylcholine receptor（α7nAChR）,nuclear factor kappa Bp65（NF-кBp65） and signal transducer and activator of transcription 3（STAT3） in brain after cerebral ischemia-reperfusion in rats with a view on cholinergic anti-inflammatory pathway.Methods： Adult male SD rats were divided randomly into 5 groups.Rats in puerarin group and α-bungarotoxin（α-BGT） group were administered intravenously with puerarin,while nicotine group were given intravenously with nicotine.After 5 days of pretreatment,the middle cerebral artery occlusion model was established according to Zea Longa＇s method.Rats in α-BGT group were injected intravenously with α-BGT immediately when the occlusion was conducted.After 24 hours of reperfusion,the infarct areas were assessed.And RT-PCR were performed to examine the mRNA expression of α7nAChR,NF-кBp65 and STAT3 in ischemic brains.Results： Compared with model group,nicotine and puerarin could reduce the infarct areas of the brain,enhance mRNA expression of α7nAChR and STAT3 in the brains of cerebral ischemia-reperfusion rats,low mRNA expression of NF-кBp65（P0.01,P0.05） significantly.While α-BGT could block the above effects of puerarin.Conclusion： Puerarin has beneficial effect of anti-inflammation in animal model of cerebral ischemia-reperfusion,and the underlying mechanism may be related to activating cholinergic anti-inflammatory pathway.

关 键 词：葛根素 脑缺血再灌注 核转录因子-кBp65 信号转导子与转录激活子3 Α7烟碱型乙酰胆碱受体 胆碱能抗炎通路 

分 类 号：R285.5[医药卫生—中药学]