运动对胰岛素抵抗小鼠PPAR-γ、Glut-4表达的影响(英文)  

Influence of exercise on expressions of PPAR-γand Glut-4 in mice with insulin resistance

作　　者：刘杰[1] 蔡颖[2] 冯彦景[2] 刘遂心[2] 

机构地区：[1]湖南中医药高等专科学校,湖南株洲412012 [2]中南大学湘雅医院心血管康复中心

出　　处：《心血管康复医学杂志》2012年第6期566-571,共6页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：湖南省科技厅基金课题(06SK3090);湖南省卫生厅基金课题(B2006-036)~~

摘　　要：目的:观察运动对高脂饮食诱导的,产生胰岛素抵抗的小鼠骨骼肌组织过氧化物酶体增殖物激活受体-γ(PPAR-γ)、葡萄糖转运体-4(Glut-4)表达的影响,初步探讨游泳训练改善胰岛素抵抗的作用机制。方法:将30只8周龄健康雄性C57BL/6J小鼠随机分为三组:正常饮食组(10例)、高脂饮食组(10例)和高脂饮食+运动组(运动组,10例,进行为期12周的游泳训练)。每周测小鼠体重和空腹血糖(FBG)。游泳训练12周后,以放射免疫法测空腹血胰岛素(FINS),并计算胰岛素抵抗指数(IRI);逆转录聚合酶链式反应法检测骨骼肌组织PPAR-γ、Glut-4 mRNA的表达。结果:与正常饮食组比较,高脂饮食组体重明显增加;运动组较高脂饮食组体重明显减轻(P<0.05)。高脂饮食组和运动组FINS、FPG、IRI水平均较正常饮食组明显升高(P<0.01)。与高脂饮食组相比,运动组FINS[(14.00±7.12)mmol/L比(10.17±3.88)mmol/L]、FPG[(9.49±1.28)mmol/L比(8.03±1.67)mmol/L]、IRI[(1.47±0.38)比(1.06±0.27)]水平明显降低(P<0.05),PPAR-γ[(0.95±0.17)比(2.37±0.41)]、Glut-4 mRNA[(0.68±0.24)比(1.54±0.28)]表达明显增加(P<0.01)。结论:运动可明显改善胰岛素抵抗,其机制可能与上调骨骼肌PPAR-γ及Glut-4 mRNA的表达,调节糖代谢,促进胰岛素信号转导有关。Objective： To observe influence of exercise on expressions of peroxisome proliferators-activated receptor-7 （PPAR-7） and glucose transporter-4 （Glut-4） in skeletal muscle tissue of mice with insulin resistance （IR） induced by high fat diet, and preliminarily investigate mechanism of swimming training improves IR. Methods： A total of 30 eight-week-old healthy male C57BL/6J mice were randomly divided into normal diet group （n= 10）, high fat diet group （n= 10） and high fat diet ＋ exercise group （HE group, n= 10, mice received 12-week swimming training）. Body weight and fasting blood glucose （FBG） of mice were measured every week. After 12-week swimming training, fasting insulin （FINS） was measured by radioimmunoassay and 1R index （IRI） was calculated; expressions of PPAR-7 and Glut-4 mRNA in skeletal muscle tissue were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results： Compared with normal diet group, body weight significantly increased in high fat diet group; body weight of HE group was significantly lower than that of high fat diet group （P〈0.05）. Compared with normal diet group, there were significant increase in FINS, FBG and IRI in high fat diet group and HE group （P〈0.01）. Compared with high fat diet group, there were significant decrease in FINS [ （14.00 ± 7.12） mmol/L vs. （10.17 ± 3.88） mmol/L], FBG [（9.49±1.28） mmol/L vs. （8.03±1.67） mmol/L]and [RI [（1.47±0.38） vs. （1.06± 0.27）, P〈0.05 all], and significant increase in expressions of PPAR-y[ （0.95±0.17） vs. （2.37±0.41）land Glut- 4 mRNA [-（0.68±0.24） vs. （1.54±0.28）, P〈0.01 both] in HE group. Conclusion：Exercise may significantly improve insulin resistance, and the mechanism may be related with upregulation of expressions of PPAR-y and Glut-4 mRNA in skeletal muscle, regulation of glucose metabolism and promotion of transduction of insulin signal.

关 键 词：运动 胰岛素 过氧化物酶体增殖物激活受体 单糖转运蛋白质类 

分 类 号：R587.102[医药卫生—内分泌]