前列地尔后处理对离体大鼠缺血再灌注损伤心肌的影响  

Influence of alprostadil postconditioning on isolated ischemia-reperfusion injured myocardium of rats

作　　者：黄炜[1] 夏勇[1] 燕宪亮[2] 马延峰[1] 

机构地区：[1]徐州医学院附属医院心血管内科,江苏徐州221002 [2]徐州医学院附属医院急救中心,江苏徐州221002

出　　处：《心血管康复医学杂志》2012年第6期589-593,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:采用Langendorff离体心脏灌注模型,探讨前列地尔后处理对离体大鼠缺血再灌注损伤心肌的影响及其机制。方法:24只SD大鼠随机分为:缺血再灌注损伤组(IR组)、前列地尔预处理组(ALP-PreC组)、前列地尔后处理组(ALP-PostC组),每组8只。检测平衡液灌注末及再灌注后不同时间点心功能、冠脉流出液及再灌注末心肌组织中生化指标和心肌梗死面积百分比。结果:平衡末,冠脉流出液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肿瘤坏死因子(TNF)-α含量,各组间无显著性差异(P均>0.05),再灌注后各组LDH、CK、TNF-α均有显著增加,再灌注后ALP-PreC组和ALP-PostC组各时间点的CK、LDH、TNF-α释放量均明显低于IR组(P<0.05)。ALP-PreC组和ALP-PostC组超氧化物歧化酶(SOD)活性显著高于IR组(P<0.01),丙二醛(MDA)含量低于IR组(P<0.05)。ALP-PreC组和ALP-PostC组心肌梗死面积百分比明显低于IR组(34.48%、32.84%比39.29%,P<0.05),ALP-PreC组和ALP-PostC组之间差别无显著性(P>0.05)。结论:前列地尔后处理可以对缺血再灌注心肌发挥保护作用,其机制可能与抑制早期炎症反应,抑制再灌注后氧自由基的过量生成,增强心肌抗氧化能力,从而发挥保护作用。Objective： To explore influence of alprostadil postconditioning on isolated ischemia- reperfusion （IR） in- jured myocardium of rats and its mechanism using Langendorff isolated heart perfusion model. Methods： A total of 24 SD rats were randomly divided into IR injury group （n=8, IR group）, alprostadil preconditioning group （n = 8, ALP- PreC group） and alprostadil postconditioning group （n = 8, ALP- PostC group）. Cardiac function and coronary effluent at the perfusion end of balancing solution and different time point after reperfusion; biochemical indexes in myocardial tissues and percentage of myocardal infarction size at the end of reperfusion were measured. Results： At the perfusion end of balancing solution, there were no significant difference in concentrations of lactate dehydrogenase （LDH）, creatine kinase （CK） and tumor necrosis factor （TNF） - a in coronary effluent among three groups （P〉0. 05 all）. After reperfusion, levels of LDH, CK and TNF - a all significantly increased in all groups, release amounts of them in ALP- PreC group and ALP- PostC group were significantly lower than those of IR group at each time point after reperfusion, P〈0. 05 all. Compared with IR group, there was significant increase in activity of superoxide dismutase （SOD, P〈0. 01） and significant decrease in malonyl diadehyde （MDA） concentration （P〈0.05） in ALP- PreC group and ALP- PostC group. Percentage of myocardial infraction size in ALP- PreC group and ALP- PostC group were significantly lower than that of IR group （34. 48%, 32.84% vs. 39.29%, P〈0. 05）, but there was no significant difference between ALP- PreC group and ALP- PostC group （P〉0. 05）. Conclusion： ALP postconditioning can protect ischemia reperfusion injured myocardium, whose mechanism may be related with inhibition of early inflammatory reaction and over production of oxygen radicals after reperfusion, and enhance myocardial antioxidant capacity.

关 键 词：前列地尔 心肌缺血 心肌再灌注损伤mg prot 

分 类 号：R969[医药卫生—药理学]