鄱阳湖区血吸虫病人免疫状态研究Ⅱ血吸虫病人细胞免疫特征分析  

Study on immune status of patients with schistosomiasis japonica in Poyang Lake region Ⅱ Characterization of cellular immune in schistosomiasis patients

作　　者：袁敏 谢曙英 周小娟[2] 胡飞 刘跃民 曾小军 李召军 李宜峰 高祖禄 李剑瑛 陈红根 林丹丹 

机构地区：[1]江西省寄生虫病防治研究所,南昌330046 [2]江西省九江学院附属医院

出　　处：《中国血吸虫病防治杂志》2012年第6期632-635,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30960344);国家科技支撑计划(2009BAI78B07);江西省科技支撑计划(2010ZDS00300)

摘　　要：目的观察鄱阳湖区血吸虫病低度流行区日本血吸虫病人细胞免疫特征。方法以鄱阳湖区血吸虫病流行区粪检阳性病人为研究对象,并以连续3年粪检阴性人群为对照,采用流式细胞术检测外周血CD4+、CD8+T细胞及CD4+CD25+Treg细胞。以可溶性血吸虫成虫抗原(SWA)和可溶性虫卵抗原(SEA)分别刺激外周血单个核细胞,采用酶联免疫吸附试验检测培养上清中IFN-γ和IL-10水平。结果日本血吸虫粪检阳性人群CD4+CD25+Treg细胞水平为(5.99±1.60)%,高于粪检阴性人群的(5.04±1.64)%,差异有统计学意义(t=3.261,P<0.01)。粪检阳性人群治疗1年后,IFN-γ水平显著提高,SEA刺激下,治疗前、后平均水平分别为121.97、2 564.03 pg/ml;SWA刺激下,治疗前、后平均水平分别为102.89、646.26 pg/ml。IL-10水平显著降低,SEA刺激下,治疗前、后平均水平分别为294.75、122.43 pg/ml;SWA刺激下,治疗前、后平均水平分别为110.10、31.85 pg/ml。结论人体感染血吸虫后诱导CD4+CD25+Treg细胞增多是引起细胞免疫应答下调的原因之一。Objective To observe the characterization of cellular immune of schistosomiasis japonica patients in low endemic area of Poyang Lake region.Methods The schistosomiasis patients who were positive by stool detections were selected as study subjects,and the individuals who were negative by stool detections in three consecutive years were selected as negative controls.The CD4＋T cell,CD8＋T cell and CD4＋CD25＋Treg in venous blood of the study subjects were detected by using flow cytometry.In vitro,cytokine（IFN-γ and IL-10） production of PBMC in response to soluble egg antigen（SEA） and soluble worm antigen preparation（SWA） were measured by ELISA.Results The levels of CD4＋CD25＋Treg in individuals who were positive by stool detections（5.99±1.60）% were higher than those of the negative（5.04±1.64）%,and the difference was significant（t = 3.261,P 0.01）.One year after the treatment,the levels of IFN-γ were significantly higher（Stimulated by SEA before and after the treatment,the mean levels were 121.97 and 2 564.03 pg/ml,respectively;stimulated by SWA before and after treatment,the mean levels were 102.89 and 646.26 pg/ml,respectively） and IL-10 were significant lower in patients who were positive by stool detections（Stimulated by SEA before and after treatment,the mean level were 294.75 and 122.43 pg / ml,respectively;stimulated by SWAP before and after treatment,the mean cytokine levels were 110.10 and 31.85 pg/ml,respectively）.Conclusion The increase of induced CD4＋CD25＋Treg is one of causes that down-regulate cellular immune of human body after infection with Schistosoma japonicum.

关 键 词：日本血吸虫病 CD4+CD25+TREG细胞 细胞免疫 鄱阳湖区 

分 类 号：R532.21[医药卫生—内科学]