光密度值标准化软件研制及若干问题探讨  

Development of a software standardizing optical density with operation settings related to several limitations

作　　者：屠小明[1] 张作恒[1] 万程[2] 郑宇[1] 徐进梅 张媛媛[4] 罗建平[1] 吴海玮 

机构地区：[1]南京医科大学基础医学院,南京210029 [2]南京医科大学康达学院 [3]美思达(上海)医药开发有限公司 [4]美国内布拉斯加大学病理与微生物学系 [5]美国罗德岛医院国际卫生研究中心

出　　处：《中国血吸虫病防治杂志》2012年第6期668-671,共4页Chinese Journal of Schistosomiasis Control

基　　金："十五"国家科技攻关项目(2004BA718B05);江苏省高校哲学社会科学项目(2012SJB880059);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的针对间接酶联免疫吸附试验(ELISA)检测日本血吸虫感染结果标准化中的几个常见问题,开发一款光密度值标准化软件,规范标准化过程和结果。方法以改良光密度值标准化变换方法(I-STOD)为开发依据,以Matlab GUI为开发工具,综合I-STOD实施过程中的各个环节以及相应的处理方法进行软件开发,并用来自日本血吸虫病疫区的血清检测结果对该软件进行测试。结果成功开发了用于标准化光密度值的软件——I-STOD V1.0。该软件的运行环境为WINDOWS XP/WIN 7,所需硬盘容量约0.5 GB。用来自日本血吸虫病疫区的血清检测结果对该软件测试表明,该软件有针对性地解决了标准化过程中的标准曲线可靠性判断、样本适用范围及超范围样本稀释度确定等问题,使得I-STOD在应用上更简便、在操作上更规范。结论基于I-STOD方法开发的I-STOD V1.0应用软件操作简便,且能有效地对间接ELISA法的实验结果进行标准化。Objective To develop a software that can be used to standardize optical density to normalize the procedures and results of standardization in order to effectively solve several problems generated during standardization of in-direct ELISA results.Methods The software was designed based on the I-STOD method with operation settings to solve the problems that one might encounter during the standardization.Matlab GUI was used as a tool for the development.The software was tested with the results of the detection of sera of persons from schistosomiasis japonica endemic areas.Results I-STOD V1.0（WINDOWS XP/WIN 7,0.5 GB） was successfully developed to standardize optical density.A serial of serum samples from schistosomiasis japonica endemic areas were used to examine the operational effects of I-STOD V1.0 software.The results indicated that the software successfully overcame several problems including reliability of standard curve,applicable scope of samples and determination of dilution for samples outside the scope,so that I-STOD was performed more conveniently and the results of standardization were more consistent.Conclusion I-STOD V1.0 is a professional software based on I-STOD.It can be easily operated and can effectively standardize the testing results of in-direct ELISA.

关 键 词：日本血吸虫病 间接ELISA 标准化 I-STOD V1 0软件 I-STOD 

分 类 号：R532.21[医药卫生—内科学]