猪带绦虫六钩蚴TSO45-4B抗原FnⅢ结构域线性B细胞表位预测及鉴定  

Prediction and identification of Taenia solium oncosphere TSO45-4B antigen FnⅢ structure domain linear B cell epitopes

作　　者：王媛媛[1] 王小莉[1] 常雪莲[1] 陶志勇[1] 夏惠[1] 方强[1] 

机构地区：[1]蚌埠医学院病原生物学教研室、安徽省感染与免疫重点实验室,蚌埠233030

出　　处：《中国血吸虫病防治杂志》2012年第6期681-684,I0001,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30600518);安徽省教育厅自然科学研究项目(KJ2011B095);蚌埠医学院校级课题(BY0804、BY1001)

摘　　要：目的预测并鉴定猪带绦虫六钩蚴TSO45-4B抗原FnⅢ结构域线性B细胞表位。方法利用生物信息学在线工具分析TSO45-4B抗原FnⅢ结构域序列并预测其线性B细胞表位;采用SWISS-MODEL软件预测TSO45-4B的蛋白空间结构,并合成两条预测表位肽。采用酶联免疫吸附试验(ELISA)检测猪囊尾蚴病人血清与两条预测表位肽的免疫反应性。结果获得2个TSO45-4B抗原FnⅢ结构域线性B细胞预测表位,其中1条预测表位合成肽可为猪囊尾蚴病患者血清识别。结论成功预测并鉴定了猪带绦虫六钩蚴TSO45-4B抗原FnⅢ结构域线性B细胞表位。Objective To predict and identify Taenia solium oncosphere TSO45-4B antigen FnⅢ structure domain linear B cell epitopes.Methods The B cell epitopes of TSO45-4B FnⅢ structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL.The peptides were synthesized according to the predicted linear epitopes.The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.Results Two linear B cell epitopes of TSO45-4B FnⅢ structure domain were predicted,and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.Conclusion Two linear B cell epitopes of TSO45-4B FnⅢ structure domain are predicted and one of them has been confirmed successfully.

关 键 词：猪带绦虫 六钩蚴 TSO45-4B FnⅢ结构域 B细胞表位 

分 类 号：R383.32[医药卫生—医学寄生虫学]