褪黑素逆转高血压大鼠学习记忆异常及嗅球神经发生障碍的实验研究  

Effects of melatonin on adult neurogenesis in the olfactory bulb as well as on learning and memory of renovascular hypertensive rat

作　　者：磨洁琳[1] 郭灵[1] 曾庆堂[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021

出　　处：《中华行为医学与脑科学杂志》2012年第12期1073-1076,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：广西青年科学基金项目（桂科青0728050）

摘　　要：目的探讨褪黑素对高血压大鼠学习记忆及吻侧迁移流（RMS）和嗅球神经发生的影响。方法建立肾性高血压大鼠模型，将大鼠分为高血压组、假手术组、褪黑素组、正常对照组，采用Morris水迷宫、BrdU单标与BrdU＋NT或BrdU＋GFAP双标免疫组化方法，测定各组大鼠行为、神经干细胞增殖与分化的状态。结果水迷宫实验：高血压组逃避潜伏期[（29．95±20．11）s]比正常对照组[（20．58±19．18）s]、假手术组[（22．11±20．28）s]、褪黑素组[（23．81±22．25）S]明显延长，差异具有统计学意义（P〈0．05）；在原平台象限的游泳时间，高血压组[（25．51±5．21）S]比正常对照组[（34．67±4．53）S]、假手术组[（33．56±6．12）S]、褪黑素组[（36．23±3．86）S]明显缩短，差异具有统计学意义（P〈0．05）。RMS的神经干细胞增殖：高血压组的BrdU阳性细胞数[（34．08±6．49）个／切片]比正常对照组[（43．53±7．56）个／切片]、假手术组[（46．11±4．75）个／切片]、褪黑素组[（44．30±8．29）个／切片]明显下降，差异有统计学意义（P〈0．05）。嗅球神经干细胞分化：高血压组的BrdU＋GFAP阳性细胞数[（2．45±1．32）个／切片]分别比正常对照组[（1．01±0．78）个／切片]、假手术组[（0．68±0．37）个／切片]、褪黑素组[（1．35±0．88）个／切片]明显升高，差异具有统计学意义（P〈0．05）；高血压组的Brdu＋NT阳性细胞数[（1．15±0．81）个／切片]分别比正常对照组[（1．89±0．98）个／切片]、假手术组[（1．63±1．01）个／切片]、褪黑素组[（2．30±1．22）个／切片]明显下降，差异具有统计学意义（P〈0．05）。结论褪黑素可能通过逆转神经发生障碍的方式来改善高血压诱发的学习记忆异常。Objective To investigate the possible abnormal changes of adult neurogenesis in the rostral migratory stream （RMS） , in the olfactory bulb and in learning or memory of renovascular hypertensive rat, and to evaluate the possible efficacies produced by intervention of exogenous melatonin. Methods Renovascular hypertensive rat model was established by clamping bilateral renal arteries. Rats were randomly divided into four groups with 10 rats per group： hypertensive, sham-operated, melatonin-treated and normal groups. Morris water maze was used to detect the abilities of learning and memory, and immunohistochemistry for bromodeoxyuridine （BrdU）, bromodeoxyuridine plus glial fibrillary acidic protein （ BrdU ＋ GFAP） and bromodeoxyuridine plus neurofilament （ BrdU ＋ NT） was utilized to examine the changes of the adult neurogenesis in RMS and in the olfactory bulb of each rat. Results The escape latency of hypertensive group （ （29.95 ± 20.11 ） s） recorded in Morris water maze test was the longest in a significant comparison to those of normal group（ （20.58 ± 19.18） s）, sham-operated group （ （ 22.11 ± 20.28 ） s ） and melatonin-treated group （ （ 23.81 ± 22.25 ） s ） （P 〈 0.05 ） ; while the time spent by rats during swimming in the quadrant with being originally put platform of hypertensive （ （25.51± 5.21 ） s） was the shortest by significant comparison to those of normal （ （ 34.67 ± 4.53 ） s）, sham-operated （ （ 33.56 ± 6.12 ） s ） and melatonin-treated group （ （ 36.23 ± 3.86 ） s ） 0 （P 〈 0. 05 ）. Proliferation of neural stem cells （cells/field） ： the num- ber of BrdU immunoreactive （BrdU-IR） cells of rats in RMS of hypertensive group （34.08 ± 6.49） was the smal- lest, significantly compared with those of normal（43.53 ± 7.56）, sham-operated （ 46.11 ± 4.75 ） and melatonin- treated group（44.30 ± 8.29 ）, （P 〈 0.05 ）. Differentiation of neural stem cells （cells/slice） ： the number of B

关 键 词：褪黑素 高血压 学习记忆 吻侧迁移流 嗅球 

分 类 号：R285.5[医药卫生—中药学]