耐高浓度铬(Ⅵ)细菌的分离筛选和鉴定  

Isolation,Screening and Identification of the Bacteria with Tolerance of the High-concentration Cr(Ⅵ)

作　　者：沈文静[1] 孟军[1] 刘标[1] 何跃[2] 

机构地区：[1]环境保护部南京环境科学研究所国家环境保护生物安全重点实验室,江苏南京210042 [2]环境保护部南京环境科学研究所国家环境保护土壤环境管理与污染控制重点实验室,江苏南京210042

出　　处：《安徽农业科学》2013年第1期238-240,共3页Journal of Anhui Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务专项

摘　　要：[目的]筛选耐高浓度Cr(Ⅵ)的细菌。[方法]以江苏某电镀厂排污口含Cr(Ⅵ)污泥为分离对象,采用选择压力法分离筛选出一批耐Cr(Ⅵ)细菌,测定分离得到的菌株耐不同浓度Cr(Ⅵ)的能力,并对9株高耐Cr(Ⅵ)细菌的分类地位进行了确定。[结果]从Cr污染土壤中筛选出26株能够耐受Cr(Ⅵ)的细菌,其中有9株细菌能够耐受300 mg/L及以上浓度的Cr(VI)。常规生理生化试验和16S rDNA测序结果表明,这9株细菌分别属于下列6个属:Arthrobacter、Bacillus、Ochrobactrum、Microbacterium、Amycolatopsis、Pusillimonas。[结论]这9株细菌在Cr(Ⅵ)污染生境的修复中存在潜在的应用价值。[ Objective ] The research aimed to screen bacteria with tolerance on high-concentration Cr （VI）. [ Method ] Mud with Cr （VI） at drain of one electroplate factory in Jiangsu as isolation object, a batch of bacteria with tolerance on Cr （VI） were screened by selection pres- sure method. Tolerance capacities of the isolated bacteria on different-concentration Cr （VI） were measured. Taxonomic status of the 9 bacte- ria was determined. [ Result ] 26 bacteria with tolerance on Cr （ V1 ） were screened from Cr-polluted soil, and 9 bacteria could endure Cr （VI） whose concentration was equal to or more than 300 mg/L. Physiological and biochemical tests and 16S rDNA sequencing result showed that these 9 bacteria respectively belonged to below 6 genera： Arthrobacter, Bacillus, Ochrobactrum, Microbacterium, Amycolatopsis and Pusillimonas. [ Conclusion] These 9 bacteria had potential application values in restoration of the Cr （VI） -polluted habitat.

关 键 词：CR(VI) 细菌 分离 筛选 鉴定 

分 类 号：S181.3[农业科学—农业基础科学]