双歧杆菌奶粉对幼儿肠道内环境影响  

THE IMPROVEMENT OF VIFIDUS MILK POWDER CONTATINING BIFIDOBACTERIUM BREVE ON INTESTINAL ENVIRONMENT OF CHILDREN

作　　者：李雪驼[1] 邱华[2] 殷素兰 王新艳 刘军 刘红延[4] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]日本医科大学客座研究员 [3]旅顺人民医院 [4]大连市中医医院药剂科

出　　处：《中国微生态学杂志》2000年第3期180-183,共4页Chinese Journal of Microecology

摘　　要：将分离到的短型双歧杆菌 CT5 0 6 6制成菌粉 ,同脱脂奶粉及 L actosucrose混合后即为试验用“双歧奶粉”,其中含短型双歧杆菌 CT5 0 6 6 :10 8CFU / g、L acto- sucrose:6 0 mg/ g。给 8名婴幼儿 (1.0～ 6 .0岁 ,离乳 )每天服用 2 5 .0 g。分别于服用前及服用 1周后、2周后及服用结束 1周后 ,检测服用儿肠内细菌群及腐败产物的变化。双歧杆菌数由服用前的 9.99± 0 .16 (log(CFU ) / g)升高到 2周后的 10 .6 1± 0 .16 ,有十分显著性差异。双歧杆菌数占肠内细菌总数的百分率由服用前的 (2 2 .1± 10 .2 ) %升高到 2周后的 (5 2 .6±12 .2 ) % ,有显著性差异。腐败菌数亦显著下降 ,与服用前相比有显著性差异。粪便中的短链脂肪酸尤其是乙酸及乳酸量显著升高 ,分别由服用前的 (2 35 .5 1± 41.3)μmol/ g、(77.15± 44 .4)μmol/ g升高到服用 2周后的 (311.5 5± 30 .8)μmol/ g、(115 .12± 2 4.2 )μmol/ g,有显著性差异。粪便中的腐败产物显著降低 ,有非常显著性差异。服用 2周后粪便中的吲哚、粪臭素等的含量与服用前相比显著降低 ,有非常显著性差异。服用期间粪便的 p H值显著降 ,有显著性差异。上述结果表明 ,服用“双歧奶粉”后 ,8名服用儿的肠内细菌群及肠道内环境得到改善。In this experiment,the Bifidus milk powder containing 10 8CFU/g Bifidobacterium breve CT5066 that isolated from healthful baby and 60mg/g Lactosucrose was administrated on 8 volunteers(1.0-6.0 years old, Weaning(or bottle-fed as feeding)) 25g/day dissoled by water lowing 40℃ being drunk one or two times a day two weeks, sampling feces before intake and after 7 days, during intake 7 days and 14 days, and then intestinal flora and putrefactive metabolites, pH, water content were measured.On intestinal flora, bifidobacteria showed increaseing significantly from (9.99±0.16) log CFU/g to 10.41±0.34 after administrating Bifidus milk powder 7 days (p<0.05) and 10.61±0.13 after 14 days(p<0.01),Meanwile,lecithinase-postive Clostridia decreased in numbers significantly(p<0.05). Ammonia showed decreaseing significantly (p<0.01);Sulfide reduced significantly at p<0.05 after administrating 14 days. Fecal pH falt down during administrating significantly (p<0.05).Fecal short chain fatty acid showed increaseing significantly, espcially acetic acid and lactic acid from (235.51±41.3) 、(77.15±44.4) μmol/g to (311.55±30.8)、(115.12±24.2) μmol/g after administrating 14 days.

关 键 词：双歧奶粉 肠内细菌群 肠道内环境 婴幼儿 

分 类 号：R333.3[医药卫生—人体生理学] TS252.51[医药卫生—基础医学]