柯萨奇B_(3m)病毒致低硒BALB/C成年鼠心肌损伤特点实验研究  

Study on CVB_(3m) caused myocardial injury in Se - deficient adult BALB/C mice

作　　者：钟学宽[1] 张邻杰[2] 于新[3] 周令望[1] 迟月明[1] 刘红[1] 万汇涓[1] 刘阳[1] 高彦辉[1] 刘艺[1] 许永莉 曾宪惠[1] 

机构地区：[1]哈尔滨医科大学克山病研究所,哈尔滨150086 [2]哈尔滨医科大学第二附属医院 [3]哈尔滨医科大学物理教研室

出　　处：《中国地方病防治》2000年第3期133-136,共4页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金课题39670650;卫生部科学研究基金96-1-3

摘　　要：应用低硒和补硒合成饲料喂养５周龄ＢＡＬＢ／Ｃ雄性小鼠５周后，经腹腔接种柯萨奇Ｂ３ｍ病毒（ＣＶＢ３ｍ）１０２ＴＣＩＤ５０，１ｍｌ对照组腹腔注射ＰＲＭ１６４０，光镜下低硒病毒组（Ｉ组），补硒病毒组（Ⅱ组）病变检出率分别为 ７５％和 ３５％，经 Ｘ２检验 Ⅰ组显著高于Ⅱ组（ Ｐ＜ ０． ０５）。且病变部位不同，低硒病毒 Ⅰ组病变主要位于左心室中层，大体标本未见心肌外膜白斑。Ⅱ组见于心外膜及心外膜下心肌，大体标本可见心外膜白斑。补硒对照组（Ⅲ）无病变。电镜结果（ Ｉ）组超微结构病变明显重于 Ⅱ组。提示硒缺乏可加重病毒感染引起的心肌病变。Male BALB/C mice(5 -week old) were randomly divided into I expermental group, Ⅱ control group and were fed Se - deficient and Se - normal food respectively. After 5 weeks, mice in group I were intraperitoneally inject- ed CVB3m. 103 TCID50 by the dosage of 0. 1 ml as well as mice in group Ⅱ with the same dosage 1640 liquor. Pathological changes of mycoardial issues were observed through LT and ET after fore were killed 7 days later original injec- tion. The results showed that, by LT, detection rates of pathological change between mice in group I (75% ) and group Ⅱ (35% ) are obviously different (X2 clack P < 0. 05), then also, the pathological location, group I, main ly occurred at middle layer of left ventricle and group Ⅱ, at epicardium and myocardium next to it. At the same time, white spot on epicardium can be observed in group I mice against the negative result, in group Ⅱ More serious ultrastructure pathological changes in group I indicates that Se - deficiency may play an important role in aggravate the pathological change of virus infected myocardium.

关 键 词：低硒 心肌炎 柯萨奇病毒 实验研究 克山病 

分 类 号：R542.21[医药卫生—心血管疾病] R542.302[医药卫生—内科学]