慢性淋巴细胞增殖性疾病83例免疫表型分析  

THE IMMUNOPHENOTYPING ANALYSIS OF 83 CASES OF CHRONIC LYMPHOPROLIFERATIVE DISORDERS

作　　者：秦文华 王桂芝 

机构地区：[1]中平能化集团总医院病理科,河南省平顶山市467000

出　　处：《中国煤炭工业医学杂志》2012年第12期1852-1856,共5页Chinese Journal of Coal Industry Medicine

摘　　要：目的研究慢性淋巴细胞系统增殖性疾病的免疫表型特点。方法采用多参数流式细胞术分析了83例慢性淋巴细胞系统增殖性疾病的免疫表型。结果 38.5%(32/83)患者共表达CD5和B细胞标志。采用WHO引用的计分系统,将患者分为B-慢性淋巴细胞白血病(B-CLL),毛细胞白血病(HCL)和其他B淋巴细胞增殖性疾病。典型的B-CLL表达CD5、CD23、CD20、CD19、HLA-DR,但仍有部分患者表达CD22、CD11c、CD25和FMC7。CD103似为HCL最特异的标志。但仅仅依靠免疫表型难以鉴别非典型B-CLL、B细胞-幼淋巴细胞白血病(B-PLL)和外套细胞淋巴瘤(MCL),细胞遗传学或分子生物学检查将有助于鉴别诊断。结论免疫表型分析是诊断慢性淋巴细胞系统增殖性疾病非常有用的依据。Objective To investigate the immunophenotype characteristic of chronic lymphoprolifera- tive disorders. Methods Multiparameter flow cytometry were employed to analyze 83 cases of chronic lym- phopro - liferative disorders. Results 38.5% （32/83）of cases co- expressed CD5 and B cell markers. The patients were classified into category of B cell chronic lymphocytic leukemia（B- CLL）, hairy cell leukemia （HCL） and other B- cell lymphoproliferative disorders（LPDs） by using the scoring system that was recom- mended by world health organization（WHO）. Typically B- CLL expressed some cytokines such as CD5 ＋ , CD23 ＋ ,CD20 ＋ ,CD19 ＋ , HLA- DR ＋ ,but the CD22, CDllc,CD25 and FMC7 were variable present in some B- CLL cases. CD103 seemed the most specific marker for HCL. To differentiate diagnosis of atypi- cal B- CLL with B- prolymphocytic leukemia （B- PLL）or mantle cell lymphoma（MCL）one must not rely exclusively on immunophenotypic dates,cytogenetic or molecular biology detection would be helpful. Conclusion Immunophenotypic analysis is an extremely useful adjunct in the diagnosis of chronic lymphoprolif- erative disorders.

关 键 词：慢性淋巴细胞增殖性疾病 免疫分型 流式细胞术 

分 类 号：R4[医药卫生—临床医学]