壳寡糖对烟草赤星病病原菌的抑制作用  

Inhibition activites of oligochitosan against alternaria alternate of tobacco

作　　者：胡国元[1] 胡坚[1] 李超影[1] 杨春雷 袁军[1] 余君 杨锦鹏 

机构地区：[1]武汉工程大学化工与制药学院,绿色化工过程教育部重点实验室,湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430074 [2]湖北省烟草科研所,湖北武汉430030

出　　处：《武汉工程大学学报》2012年第12期4-7,共4页Journal of Wuhan Institute of Technology

基　　金：武汉工程大学研究生教育创新基金项目(CX201119)

摘　　要：探讨了壳寡糖对烟草赤星病病原菌(链格孢菌)菌丝生长、孢子萌发、产孢量的影响,并研究了温度和pH值对壳寡糖抑菌作用的影响.结果表明:浓度为15g/L的壳寡糖在48h对烟草赤星病病原菌菌丝生长的抑制率为94.74%.壳寡糖抑制病原菌孢子萌发能力强于抑制病原菌菌丝生长,浓度为2.5g/L的壳寡糖对病原菌孢子萌发抑制率达到100%.质量浓度为10g/L的壳寡糖在144h对链格孢菌产孢抑制率为69.7%.壳寡糖的抑菌效果具有良好的热稳定性,pH值对抑菌效果影响较大,质量浓度为15g/L的壳寡糖在48h、培养基pH为4时对菌丝生长抑制率达到100%,培养基pH为7时抑制率为62.26%.The effects of oligochitosan on mycelial growth, spore germination and sporulation of Alternaria alternate of tobacco were explored, and the effects of temperature and pH values to the antibacterial effect of oligochitosan were also studied. The results show that when the concentration of oligochitosan is 15 g/L, the inhibition rate of mycelial growth to mycelial growth of A. alternate is 94.74% in 48 h, and when the concentration of oligochitosan is only 2.5 g/L, the inhibition rate to spore germination of A. alternate is 100%. When the concentration of oligochitosan is 10 g/L, the inhibition rate of sporulation of A. alternate is 69.7% in 144 h. The antibacterial effect of oligochitosan has a good thermal stability. The pH values show great influence to the antibacterial effect of oligochitosan. When the concentration of oligochitosan is 15 g/L, the inhibiton rates of mycelial growth of A. alternate is 100% with the medium pH 4 in 48 h, and 62.26% with the medium pH 7 in 48 h.

关 键 词：壳寡糖 烟草赤星病 链格孢菌 抑制作用 

分 类 号：Q939.95[生物学—微生物学]