根癌农杆菌介导的玉米自交系A188愈伤组织再生条件的优化  

Optimization of Conditions for Agrobacterium tumefciens-Mediated Transformation and Regeneration of Maize Inbred Line A188 Callus

作　　者：仇平平[1] 辛相启[2] 刘文浩[1] 苏文敏[1] 竺晓平[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省农业科学院植物保护研究所,山东济南251000

出　　处：《山东农业科学》2012年第12期28-31,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金(30871620);国家转基因专项子课题(2009ZX08009-050B)资助

摘　　要：以A188玉米自交系的愈伤组织为材料,研究了影响农杆菌介导玉米转化和再生的各种因素,建立了该自交系的遗传转化体系。结果表明,1.0～1.5 mm的幼胚最易诱导出胚性愈伤组织;在侵染液和共培养基中加入乙酰丁香酮能显著提高转化效率;合适的菌液浓度(OD600=0.4)、共培养时间(3 d)、共培养温度(24℃)有利于提高转化效率。The factors affecting the Agrobacterium tumefciens - mediated transformation and regeneration of maize （Zea mays L. ） embryogenic callus were optimized, and the genetic transformation system was established for the maize inbred line A188. The results indicated that 1.0 - 1.5 mm immature embryos were easier to induce embryogenic callus; the addition of acetosyringone in infection solution and common medium could increase the transformation efficiency significantly; appropriate bacterial concentration （OD600 = 0.4）, incubation time （3 days） and co- culture temperature （24℃） were benefit to increasing formation efficiency.

关 键 词：玉米 根癌农杆菌 愈伤组织 再生体系 

分 类 号：S513.01[农业科学—作物学]