水库贫营养好氧反硝化细菌分离及其对水体微生物群落影响  

Isolation of oligotrophic aerobic denitrifying bacteria and its effects on the water microbial communities

作　　者：黄廷林[1] 刘婷婷[1] 张海涵[1] 杨霄[1] 郭琳[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《西安建筑科技大学学报（自然科学版）》2012年第6期876-882,共7页Journal of Xi'an University of Architecture & Technology(Natural Science Edition)

基　　金：国家科技支撑计划课题(2012BAC04B02);国家自然科学基金重点项目(50830303);陕西省科技统筹创新工程计划项目(2011KTCG03-07)

摘　　要：经过贫营养好氧反硝化选择性培养基的初步筛选,从水源水库沉积物中分离得到234株贫营养好氧反硝化细菌,并对其脱氮特性和高效菌株对水体功能微生物种群的调控机制进行探索.结果表明,8株贫营养好氧反硝化细菌在48h内脱氮率达80%以上.经过16srDNA序列分析,确定分子遗传种属为不动杆菌属、链霉菌属、嗜甲基菌属、根瘤菌、假单胞菌、鞘氨醇单胞菌和溶杆菌属等.巢式PCR-DGGE结果表明接种高效贫营养好氧反硝化细菌HF3对原水水体功能微生物(细菌、真菌、放线菌和硫酸盐还原菌)种群结构多样性影响不显著,对原水微生物生态种群影响微弱.Based on a preliminary screening,234 oligotrophic aerobic denitrifying bacterial strains grown on oligotrophic aerobic denitrifying selective medium were isolated from the drinking water reservoir sediments.Meanwhile,the denitrification characteristics and its regulation mechanism on the water microbial communities were explored.The results showed that denitrification rates of eight oligotrophic denitrifying bacteria were more than 80% within 48h.Acinetobacter,Streptomyces,Methylophilus,Rhizobium,Pseudomonas were identified using 16 s rDNA sequences.Nested PCR-DGGE results revealed that highly oligotrophic denitrifying bacteria HF3 inoculation have no significant impact on the raw water functional microbial communities（bacteria,fungi,actinomycetes and sulfate-reducing bacteria） structure diversity.Therefore,its effect on the raw water microbial ecological population is weak.

关 键 词：水库 沉积物 好氧反硝化细菌 微生物群落 巢式PCR-DGGE 

分 类 号：X703[环境科学与工程—环境工程]