谷酰胺对高体积分数氧肺损伤新生大鼠的保护作用  

Protective Effect of Glutamine on Lung Injury Induced by Hyperoxia in Neonatal Rats

作　　者：张海鸿[1] 刘秀香[1] 赵国英[1] 王宝宏[1] 郭金将[1] 罗瑶[1] 

机构地区：[1]滨州医学院附属医院儿科,山东滨州256603

出　　处：《实用儿科临床杂志》2012年第24期1891-1893,1912,共4页Journal of Applied Clinical Pediatrics

基　　金：山东省科技攻关项目(2009GG20002055);山东省优秀中青年科学家科研奖励基金(BS2010YY003);滨州医学院科研计划项目(BY2009KJ15)

摘　　要：目的探讨谷酰胺对新生大鼠高体积分数氧(高氧)肺损伤的保护作用。方法将足月2日龄新生SD大鼠24只随机均分为空气组、谷酰胺组和高氧组,每组8只。空气组新生大鼠不做处理,谷酰胺组新生大鼠实验前腹腔注射0.75 g·kg-1·d-1谷酰胺溶液,高氧组新生大鼠腹腔注射等容量9 g·L-1盐水,连续3 d;高氧组与谷酰胺组新生大鼠置于氧体积分数大于900 mL·L-1模型箱内,空气组新生大鼠置于同一室内常压空气中。分别于实验开始3 d、6 d比较各组新生大鼠体质量,于实验开始6 d无菌条件下处死新生大鼠,取肺组织,24 h后制成石蜡标本,观察病理改变及热休克蛋白70(HSP70)的表达。结果 1.高氧组6 d存活率明显低于空气组(P<0.05);谷酰胺组6 d存活率与空气组比较差异无统计学意义(P>0.05)。高氧下暴露3 d,3组体质量比较差异无统计学意义(P>0.05);高氧暴露6 d,高氧组体质量明显低于空气组(P<0.01),谷酰胺组体质量与空气组比较差异无统计学意义(P>0.05)。2.高氧组肺水肿程度最严重,其肺湿质量/干质量值明显高于空气组(P<0.05);谷酰胺组肺湿质量/干质量值与空气组比较差异无统计学意义(P>0.05)。3.高氧暴露6 d,高氧组肺泡内有出血和大量渗出的炎性细胞、大量粉染的蛋白性液体,肺泡壁严重充血水肿;谷酰胺组肺泡内极少量红细胞渗出,肺泡腔内见粉染的水肿液,肺泡壁增厚。4.高氧暴露6 d,高氧组、谷酰胺组肺组织HSP70蛋白表达量均较空气组显著增加(Pa<0.01),谷酰胺组肺组织HSP70蛋白表达量较高氧组显著增加(P<0.01)。结论预防性使用谷酰胺可明显改善高氧肺损伤新生大鼠的生存率,有利于新生大鼠的生长发育;预防性使用谷酰胺能增加高氧肺损伤新生大鼠肺组织HSP70的表达,表明谷酰胺对高氧肺损伤的保护作用与HSP70表达相关。Objective To explore the protective role of glutamine on lung injury induced by hyperoxia in neonatal rats. Methods Twenty - four 2 - day - old newborn rats were randomly divided into air group （ n = 8 ）, glutamine group （ n = 8 ） and hyperoxia group （ n = 8）, among which the rats in air group were not processed. The rats in glutamine group were processed through intraperitoneal injection of 0.75 g .kg-1 . d-1 glutamine solution before the experiment,and the rats in hyperoxia group were intraperitoneal injected with the same a- mount of 9 g .L-1 saline, and the above experiment was processed for following 3 days;the rats in hyperoxic group and glutamine group were located in the model case which had more than 900 mL . L-1 oxygen concentration, and in a same room, the rats in air group were located at atmospheric pressure in air. Through the statistical comparison, body mass of the each group at 3 days and 6 days were measured, separately. The rats were sacrificed after 6 days and lung tissue was removed to make paraffin specimen under aseptic conditions, then the expression of pathological changes and heat shock protein 70 （HSP70） were observed. Results 1. In the 6th day after experiment,the survival rate in hype- roxia group was significantly lower than that in air group （ P 〈 0.05 ）, while between the glutamine group and the air group, there was no sig- nificant difference （ P 〉 0.05 ）. Exposing to hyperoxia for 3 days, the weight among 3 groups had no significant difference （ P 〉 0.05 ）, but when they were exposed to hyperoxia for 6 days,the weight of newborn rats in hyperoxia group was significantly lower than that in air group （P 〈 0.01 ） ;however, the glutamine group＇ newborn rats had no significant difference compared with air group （P 〉 0.05 ）. 2. The pulmonary edema in hyperoxia group was the most serious, and the wet weight/dry weight values were significantly higher than those in air group （P 〈 0.05 ）. However, the wet weight/dry weight values in gl

关 键 词：谷酰胺 高体积分数氧肺损伤 热休克蛋白70 

分 类 号：R563[医药卫生—呼吸系统]