肝脾调补方对小鼠免疫性肝损伤的防护作用  

Experimental study of Ganpitiaobu Fang on immunological liver injury in mice

作　　者：阮连国[1] 张妍[2] 朱清静[1] 

机构地区：[1]武汉市医疗救治中心肝病科,湖北武汉430023 [2]武汉市妇女儿童医疗保健中心

出　　处：《中西医结合肝病杂志》2012年第6期363-365,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：武汉市卫生局资助项目(No.WX08D19)

摘　　要：目的:观察中药肝脾调补方对小鼠免疫性肝损伤的防护作用并探讨其药理学机制。方法:采用卡介苗(BCG)+脂多糖(LPS)建立小鼠免疫性肝损伤模型,通过肝脾调补方高、中、低剂量灌胃,观察肝脾调补方对各组小鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)及肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平的影响;ELISA法检测肿瘤坏死因子-α(TNF-α)的变化。结果:肝脾调补方可明显降低免疫性肝损伤小鼠血清中ALT、AST、TNF-α水平;减少肝组织匀浆的MDA含量,升高肝匀浆SOD、GSH-Px含量。结论:肝脾调补方对BCG+LPS尾静脉注射造成的小鼠免疫性肝损伤有明显的防护作用,其作用与抗脂质过氧化有关。Objective： To study the effects of Ganpitiaobu Fang in protecting immunity hepatic injury induced by bacille calmette Guerin （BCG） and lipopolyhepatic （LPS） in mice and study on its pharmacological mechanism. Methods： The immu- nological liver injury rat model was produced by intraperitonealitonealinjection with BCG plus LPS. Sixty mice divied into control group , model group, low dose group, middle dose group and high dose group randomly. The levels of alanine am intransferase （ALT）, aspartate an inotransferase （AST）, tumor necrosis factor-α （TNF-α） in blood plasma and super oxide dismutase （SOD） of liver homogenate malondialdehyde （MDA）, Glutathione peroxidase （GSH-PX） of the five groups were examined. Results： Ganpitiaobu Fang significantly reduced immunological liver injury in mice by reducing serum ALT, AST, TNF-alpha level; reducing MDA content of liver homogenate and elevated SOD, GSH-PX of contents liver homogenate. Conclu- sion： Ganpitiaobu Fang can obviously proteet immunologicalinjury to liver in rats. Its role mechanism is related with anti-lipid peroxidation.

关 键 词：免疫性肝损伤 肝脾调补方 药效学 作用机制 

分 类 号：R285.5[医药卫生—中药学]