环匹阿尼酸对大鼠远端肺静脉平滑肌细胞内钙浓度的影响  

The effects of cyclopiazonic acid on intracellular calcium cation concentration in rat distal pulmonary venous smooth muscle cells

作　　者：彭公永[1] 蒋永亮[1] 胡国平[1] 周玉民[1] 胡锦兴[1] 赵祝香[1] 邹威凤[1] 

机构地区：[1]广州医学院笫一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室,广州510120

出　　处：《广东医学》2012年第23期3511-3514,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81000020);广州市属高校羊城学者科研项目(编号:10A025G);广州市属高校科研项目(编号:10A276);呼吸疾病国家重点实验室青年科学基金支持项目(编号:11)

摘　　要：目的研究环匹阿尼酸(CPA)对大鼠远端肺静脉平滑肌细胞(PVSMC)细胞内钙浓度([Ca2+]i)的影响及可能机制。方法原代培养大鼠远端PVSMC,并通过形态学和免疫荧光染色鉴定,利用荧光显微镜和InCyte细胞内钙浓度检测系统观测高钾(60 mmol/L KCl)溶液及CPA对PVSMC的[Ca2+]i影响。结果原代培养的PVSMC表现出典型血管平滑肌细胞生长特征,并对平滑肌α-actin免疫荧光染色呈阳性反应;高钾溶液使PVSMC的[Ca2+]i显著升高,5μmol/L硝苯地平能完全阻断PVSMC的[Ca2+]i对高钾溶液的反应;10μmol/LCPA能使含5μmol/L硝苯地平的无钙Krebs溶液孵育的PVSMC的[Ca2+]i短暂小幅升高,恢复细胞外Ca2+至2.5 mmol/L后,10μmol/L CPA能使含5μmol/L硝苯地平的Krebs溶液孵育的PVSMC的[Ca2+]i迅速升高。结论 CPA能够使大鼠远端PVSMC的[Ca2+]i升高,其机制可能与Ca2+从肌浆网释放,并诱发细胞外Ca2+通过钙池操纵性钙通道(SOCC)内流有关。Objective To study the effect of cyclopiazonic acid （CPA） on intracellular calcium cation concentration （ [ Ca^2＋ ]i） in rat distal pulmonary venous smooth muscle cells （PVSMCs）. Methods The primary cultured rat distal PVSMCs were identified by morphology and immunofluorescence. The effects of high potassium （60 mmol/L） and CPA on [ Ca^2＋ ]i in PVSMC were assessed by fluorescence microscopy and InCyte [ Ca^2＋ ]i measurement system. Results Typical growth pattern of vascular smooth cell and positive a - actin staining were observed on the primary cuhureof PVSMC. There was significant increase of [ Ca^2 ＋ ] i in PVSMC in response to high potassium, which could be completely reversed by nifedipine （5 μmol/L）. In PVSMC perfused with Ca2＋ -free Krebs solution containing nifedipine, CPA （10 μmol/L） caused a small transient increase in [ Ca^2＋] i.after restoration of extracellular Ca^2＋ to 2. 5 mmol/L, CPA caused marked increases in [ Ca2 ＋ ] i. Conclusion CPA increases the [ Ca^2 ＋ ] i in rat distal PVSMC. CPA induced increase in [ Ca^2＋ ] i may related to Ca^2＋ release from sarcoplasmic reticulum and the influx of Ca^2＋ through store - operated Ca^2＋ channels （SOCC） in PVSMC.

关 键 词：环匹阿尼酸 细胞内Ca2+浓度 肺静脉平滑肌细胞 大鼠 钙池操纵性钙通道 

分 类 号：R543[医药卫生—心血管疾病]