血小板上表达的P-选择素对B16细胞分泌肿瘤坏死因子和血管内皮生长因子的影响  

The effects of platelet P-selectin on tumor necrosis factor-α and vascular endothelial growth factor secreted by B16 cells

作　　者：亓翠玲[1] 郭四美[1] 叶杰[1] 周秦[1] 郑力[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《广东医学》2012年第23期3527-3529,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30900764);广东省医学科研基金资助项目(编号:A2011299)

摘　　要：目的探讨血小板上表达的P-选择素对B16细胞分泌肿瘤坏死因子(tumor necrosis factor-α,TNF-α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响。方法将B16细胞分别与C57小鼠和P-选择素敲除小鼠的血小板共培养,用ELISA法检测上清中的肿瘤坏死因子和血管内皮生长因子的浓度。结果 B16细胞与C57小鼠的血小板共培养的血清中血管内皮生长因子的浓度明显高于与P-选择素敲除小鼠的血小板共培养的血清中的浓度,差异有统计学意义(P<0.05),而肿瘤坏死因子的浓度差异无统计学意义。结论血小板上表达的P-选择素促进了B16细胞分泌血管内皮生长因子,但对肿瘤坏死因子的分泌则没有影响。Objective To investigate the effects of platelet P - selectin on tumor necrosis factor - c~ and vascular endothelial growth factor secreted by B16 cells. Methods The B16 cells were co - cultured with the platelets from C57 mice and P - seleetin knockout mice. The TNF - a and VEGF concentrations in the supernatant were assessed using ELISA. Results The VEGF concentration was significantly higher in C57 group than that in P - selectin knockout group, though there was no significant difference in TNF - a. Conclusion Platelet P - selectin promotes B16 cells to secrete VEGF, but with no effect on TNF- a.

关 键 词：血小板 P-选择素 肿瘤坏死因子 血管内皮生长因子 

分 类 号：R286.2[医药卫生—中药学]