芒果畸形病的巢式PCR检测  

Detection of mango malformation disease by nested-PCR

作　　者：贾静[1,2] 蒲金基[2] 吕延超[2] 占魏[2] 王芳[2] 谢艺贤[2] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《广东农业科学》2012年第22期162-164,共3页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(201203092-2)

摘　　要：建立了检测芒果畸形病的有效方法。利用Fusarium proliferatum的钙调蛋白基因序列设计外侧引物PRO1/PRO2和内侧引物O1/O2,采用引物PRO1/PRO2可以从芒果畸形病病原菌基因组中扩增出527 bp的特异性基因片段,采用引物O1/O2可获得477 bp的基因片段。巢式PCR的检测灵敏度达5×10-5ng/μL,是常规PCR的100倍。此法具有良好的特异性和检测灵敏度,为芒果畸形病的早期诊断和及时有效的防治提供技术方法和理论依据。The study was to establish an effective detection technique for mango malformation disease. The outer pair of primers PRO1/PRO2 and the inner pair of primers O1/O2 were designed, according to the calmodulin gene sequences of Fusarium proliferatum. The results showed that a 527-bp specific gene fragment was obtained by PRO1/PRO2, and 477-bp was amplified by O1/O2. The sensitivity of nested-PCR was as high as to the 5×10^-5ng/μL, which was 100 times higher than the sensitivity of routine PCR. Nested- PCR was a high specificity and sensitivity to the disease, the technique and theory would be provided for early diagnosis and preventive treatment.

关 键 词：芒果畸形病 巢式PCR 检测 

分 类 号：S667.7[农业科学—果树学]