普安银鲫mtDNA D-loop序列多态性分析  

Polymorphism of D-loop sequence from mtDNA of Carassius auratus gibelio Bloch in Puan

作　　者：周其椿[1] 林艳红[1] 彭江涛[1] 王金娜[1] 王禄超[1] 安苗[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025

出　　处：《广东农业科学》2012年第22期173-175,183,共4页Guangdong Agricultural Sciences

基　　金：贵州省农业科技攻关项目(黔科合NY字[2009]3070);贵州省科学技术基金项目(黔科合J字[2011]2223);国家自然科学基金(31160527)

摘　　要：为从分子水平探讨普安银鲫遗传多样性,运用PCR扩增和DNA直接测序技术,对25尾鱼的线粒体DNA的D-loop区段序列进行分析。结果表明:80%的普安银鲫mtDNA D-loop区全序列长度为923 bp,少数个体由于碱基缺失及插入为920 bp和924 bp;其序列中A+T平均含量(53.36%)高于G+C含量(46.64%);共发现36个变异位点,定义了10种单倍型,单倍型间的平均遗传距离为0.017,单倍型多样度(Hd)为0.850,核苷酸多样性(Pi)为0.01376,平均核苷酸差异数(K)为12.660,普安银鲫存在丰富的遗传多样性。The paper analyzed the D-loop area sequence of mitochondrial DNA of 25 fish by PCR assay and DNA direct sequencing technique for investigating the genetic diversity of Carassius auratus gibelio （Bloch） in molecular field. The results showed that the whole sequence of mitochondrial DNA was 923 bp in 80% Carossius auratus gibelio （Bloch）, others were 920 bp or 924 bp as base deletion and insertion. The mtDNA D-loop zone was rich in A＋T （53.36%） compared with G＋C （46.64%）. There were 36 variable sites which delimited 10 species of haplotype. It also showed that the average genetic distance was 0.017, haplotype diversity （Hd） was 0.850, nucleotide diversity（Pi） was 0.01376, and the average amount of nucleotide difference（K） was 12.660. Carassius auratus gibelio（Bloch） has showed good genetic diversity.

关 键 词：普安银鲫 MTDNA D—loop 多态性 遗传多样性 

分 类 号：S965.117[农业科学—水产养殖]