黄皮尖椒秀黄F1种子纯度的RAPD鉴定  

Determination of seed purity of yellow skin pepper Xiuhuang F1 by RAPD

作　　者：李智军 曾晶[1,2] 龙卫平 卢文佳 朱志全[1,2] 

机构地区：[1]广东金作农业科技有限公司 [2]广东省农科院作物研究所,广东广州510640

出　　处：《广东农业科学》2012年第23期136-138,共3页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2010B050300012)

摘　　要：使用180条RAPD引物对黄皮尖椒秀黄F1及其亲本模板DNA进行PCR扩增,筛选出8条特异引物,其中2条偏父型(SPS-H2、SPS-H7)、2条互补型(SPS-V10、SPS-Z6)、4条偏母型(SPS-X3、SPS-X4、SPS-Z10、SPS-Z2)。用筛选出的引物SPS-H2对该品种一批商品种进行鉴定,发现其种子纯度为96.6%,与根据果实性状进行的田间鉴定结果(95.8%)吻合,表明RAPD技术用于种子纯度鉴定所获的结果准确可靠,可节省大量的人力、物力及时间。8 specific primers were selected from 180 RAPD primers by PCR amplification on template DNA of FI and its parents of a yellow skin pepper Xiuhuang, in which 2 primers were partial male type （SPS-H2, SPSH7 ）, 2 primers complementary type （SPS-V10, SPS-Z6）, and 4 primers partial female type（SPS-X3, SPS-X4, SPS-Z10, SPS-Z20）. Using SPS-H2, one of the seleelLed specific primers, the hybrid purity of a commercial seeds of this cultivar were determined and the purity was 96,6%, being quite consistent with that （95.8%）identified according to fruit characters in field. The results showed that the purity identification by RAPD technique was accurate and reliable, and by contrast it can save lots of manpower, material resources and time.

关 键 词：黄皮尖椒 秀黄 RAPD 纯度鉴定 

分 类 号：S641.3[农业科学—蔬菜学]