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作 者:戴国俊[1,2] 孙大辉[1,2] 王翔[1,2] 孙明明[1,2] 张跟喜[1,2] 谢恺舟[1,2] 施会强[3] 侍苗苗[1] 王金玉[1,2]
机构地区:[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]江苏京海禽业集团有限公司,江苏海门226103
出 处:《中国畜牧兽医》2012年第12期113-116,共4页China Animal Husbandry & Veterinary Medicine
基 金:国家肉鸡产业技术体系(nyctx-42-G1-05);江苏省高校自然科学研究重大项目(11KJA230001);江苏高校优势学科建设工程资助项目(PAPD)
摘 要:以京海黄鸡为试验材料,采用PCR-SSCP技术检测斑联蛋白(zyxin)基因外显子9的SNP,探讨zyxin基因该位点多态性与京海黄鸡屠宰性状之间的关系。结果发现该位点存在1个SNP突变位点,表现为3种基因型:AA、AB和BB。统计分析表明,公鸡的胸肌重AA基因型显著高于AB基因型(P<0.05),活体重和屠体重AA基因型显著高于BB基因型(P<0.05);母鸡的活体重、头重、爪重和屠体重AA基因型显著高于BB基因型(P<0.05),全净膛重AA基因型显著高于AB基因型(P<0.05)。Jinghai Yellow chickens were chosen for experimental animal,PCR-SSCP was used to detect the SNP of zyxin gene in exon 9.The correlation between this SNP genotypes of zyxin gene and slaughter performances was analyzed.The result showed that one mutation was found in exon 9 which comprised three genotypes,named AA,AB and BB.Statistics analysis showed that AA genotype was significantly better than AB genotype on pectoral muscle weight(P0.05),and AA genotype was significantly better than BB genotype on living weight as well as carcass weight in cocks(P0.05).AA genotype was significantly better than BB genotype on living weight,cerebaria,claw weight and carcass weight(P0.05),and AA genotype was significantly better than AB genotype on eviscerated chicken weight in hens(P0.05).
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