中国地鼠微卫星DNA引物的设计及筛选  

Designing and Screening of Microsatellite Primers in Chinese Hamster

作　　者：宋国华[1,2] 耿佳宁[3] 贾若愚[3] 岳文斌[2] 刘田福[1] 胡松年[3] 

机构地区：[1]山西医科大学实验动物中心,山西太原030001 [2]山西农业大学动物科技学院,山西太谷030801 [3]中国科学院北京基因组研究所,北京100029

出　　处：《中国畜牧兽医》2012年第12期128-132,共5页China Animal Husbandry & Veterinary Medicine

基　　金：山西省实验动物专项资金资助(2005K01)

摘　　要：本研究旨在筛选中国地鼠微卫星位点,为中国地鼠遗传分析提供遗传标记。根据建立的中国地鼠微卫星富集文库,对筛选的含微卫星DNA序列的克隆应用引物设计软件Primer 3.0设计引物135对,选择合成11对理论上易出现影子带的引物,用20个中国地鼠个体对这些引物进行了评估。结果显示,11对引物均能扩增出谱带,这11条带的平均多态信息含量(PIC)为0.4195,平均观察杂合度(Ho)为0.3895,平均期望杂合度(He)为0.4565,每个位点的平均等位基因数为5.818。筛选出的微卫星位点均可用于中国地鼠种群遗传结构分析,这将为中国地鼠品种选育、种系评估提供更多的微卫星遗传标记信息。The objectives of the present study were to screen new microsatellite loci in Chinese hamster to develop genetic markers for genetic analysis.A library of partial small size fractionated genomic DNA was constructed with the Chinese hamster.135 pairs of primers were designed with the software Primer 3.0 for microsatellites positive clones obtained.11 pairs primers that the stutter bands easily in theory were synthesized and 20 samples were tested with them.The results showed 10 of the 11 loci were found to be polymorphic,and PIC,the mean observed heterozygosities（Ho）,the mean expected heterozygosities（He） were 0.4195,0.3895 and 0.4565, respectively.The microsatellite markers would be useful for further studying on accessions identification and breeding of Chinese hamster,which would provide some evaluable tools for marker-assisted selection breeding and gene mapping in Chinese hamster.

关 键 词：中国地鼠 微卫星 基因组文库 

分 类 号：S813.3[农业科学—畜牧学]