基于线粒体DNA控制区(mtDNA D-loop)序列分析贵妃鸡的遗传多样性  

Genetic Diversity of Royal Chicken Basesd on Mitochondrial DNA D-loop Region Sequence

作　　者：吴婵[1,2] 李辉[2] 李敬瑞[2] 张大权 谢国午 孔嫣 易恒洁[2] 

机构地区：[1]贵州省晴隆县草地畜牧中心,贵州晴隆561400 [2]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025

出　　处：《中国畜牧兽医》2012年第12期137-140,共4页China Animal Husbandry & Veterinary Medicine

基　　金：贵州大学引进人才基金(Z065052)

摘　　要：采用PCR产物直接测序技术对30只贵妃鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。结果表明,在所分析的D-loop区部分序列中(520 bp),A、G、C、T平均含量分别为26.8%、13.0%、31.1%和29.1%;共发现13个核苷酸多态位点,均为转换位点,未检测到插入/缺失和颠换,核苷酸多样度(Pi)为0.0059,单倍型变异度(Hd)为0.538,中性检验Tajima’s D值为-0.67065。通过群体构建的NJ聚类图分子系统树发现,贵妃鸡起源于红原鸡。In this paper,the mitochondrial DNA D-loop sequence（520 bp in length） of 30 individuals from Royal chicken of the first Ⅰ region sequences were analyzed by PCR product direct sequencing technology.The results revealed that the average values of base composition of A,G,C and T in the sequence were 26.8%,13.0%,31.1% and 29.1%,respectively.And there were 13 nucleotide polymorphic sites which were transition neither transversion nor insertion/deletion.The haplotype diversity（Hd） and average nucleotide diversity（Pi） of the sites were 0.538 and 0.0059,and the value of Tajima’s D was-0.67065.Using the NJ method showed that the Royal chicken origined in the Red Gallus.

关 键 词：贵妃鸡 线粒体DNA D-LOOP 遗传多样性 单倍型 

分 类 号：S816.79[农业科学—饲料科学]