CDKN2、nm23基因产物的表达与卵巢上皮性肿瘤生物学行为的关系  

Relation of CDKN2、nm23 Gene Products Expression with Biologic Behavior of Ovarian Epithelial Tumor

作　　者：王丹波[1] 姜彦多[2] 马晓欣[1] 

机构地区：[1]中国医科大学第二临床学院妇产科,沈阳110003 [2]沈阳军区第二○二医院病理科

出　　处：《中国医科大学学报》2000年第3期226-228,共3页Journal of China Medical University

摘　　要：目的 :p16蛋白和 NDPK/ nm2 3在不同类型、不同分期的卵巢上皮性肿瘤的表达情况。方法 :采用 S- P法免疫组化染色观察 p16蛋白和 NDPK/ nm2 3在 37例卵巢良性肿瘤、33例卵巢交界性肿瘤和 6 9例卵巢上皮癌的表达情况。结果 :在卵巢癌中 ,p16蛋白表达阳性率降低 (5 0 .7% ,P <0 .0 1) ;NDPK/ nm2 3表达阳性率增高 (73.9% ,P <0 .0 5 )。p16蛋白表达与组织学类型无关 (P >0 .0 5 ) ;而 NDPK/ nm2 3在粘液性癌表达阳性率 (5 4.5 % )与浆液性癌 (82 .2 % )和子宫内膜样癌 (83.3% )比较 ,差异显著 (P <0 .0 5 )。 p16蛋白和 NDPK/ nm2 3在不同分化程度卵巢癌中的表达无显著差异。两基因产物在 ～ 期癌表达阳性率 (p16蛋白 :6 8.0 % ;NDPK/ nm2 3:88.0 % )均高于 ～ 期癌 (p16蛋白 :38.7% ;NDPK/ nm2 3:6 4.5 % ) (P <0 .0 5 )。结论 :抑癌基因 CDKN2的基因产物 p16蛋白表达缺失在卵巢癌的发生和发展中可能都起重要作用 ;而 nm2 3基因的过度表达对卵巢癌的发生起重要作用 。Objective: Our purpose was to study the expression of p16 protein and NDPK/nm23 in ovarian epithelial tumor. Methods:Compared with normal ovary, the expression of p16 protein and NDPK/nm23 was observed immunohistochemically on formalin fixed paraffin embedded sections in 37 cases of ovarian benign tumor, 33 cases of ovarian borderline tumor and 69 cases of ovarian epithelial carcinoma. Results:The positive rate of p16 protein was lower(50.7%,P<0.01) and that of NDPK/nm23 was higher(73.9%, P<0.05) in ovarian carcinoma. The present of p16 protein was not related to histological types of tumor. The mucous carcinoma (54.5%) differed significantly from serous (82.8%) and endometrioid (83.3%) carcinoma in positive rate of NDPK/nm23 (P<0.05) . Among ovarian carcinoma,the expression of p16 protein and NDPK/nm23 was not related to differentiation of the tumor.The positive rates of both gene products were higher in stageⅠ to Ⅱcarcinoma (p16 protein: 68.0%; NDPK/nm23: 88.0%) than in stage Ⅲ to Ⅳ carcinoma (p16 protein: 38.7%; NDPK/nm23: 64.5% ) (P<0.05) . Conclusion: Inactivation of tumor suppressor gene CDKN2 which leads to deletion of p16 protein expression are involved in the genesis and progression of ovarian carcinoma, nm23 gene might profit more with the genesis and less with the progression of ovarian carcinoma.

关 键 词：卵巢肿瘤 免疫组织化学 P16蛋白 NM23基因 

分 类 号：R737.81[医药卫生—肿瘤]