山东省部分地区献血员丙型肝炎病毒感染状况及分型研究  

A Study on Prevalence and Subtypes of Hepatitis C Virus among Blood Donors in Some Areas of Shandong Province

作　　者：刘淑贞[1] 傅继华[1] 崔怡辉[2] 庄辉[2] 刘传新[1] 石长胜 苏生利[1] 

机构地区：[1]山东省卫生防疫站,济南250014 [2]北京医科大学微生物学系 [3]山东省泰安市卫生防疫站

出　　处：《中国公共卫生》2000年第6期527-528,共2页Chinese Journal of Public Health

摘　　要：选择 42 4例有偿献血员用ELISA检测抗 -HCV ,对抗 -HCV阳性血清用ELISA进行血清学分型 ,对己确定血清型者采用RT—PCR作基因分型。结果 42 4例献血员中 ,HCV感染率为 2 1 70 % ( 92 / 4 2 4) ,献全血并血浆者的HCV感染率( 45 14% )显著高于只献全血者 ( 6 94% ) ;92例抗—HCV阳性者中 ,血清学分型率为 34 78% ( 32份 ) ,其中血清学Ⅰ型 30例( 93 75 % )、Ⅱ型 2例 ( 6 2 5 % ) :该 32例血清中有 30例可用基因分型 ,其中基因Ⅱ型 2 6例 ( 86 6 7% )、Ⅲ型 3例 ( 10 % )、Ⅱ /Ⅲ型混合感染 1例 ( 3 33% ) ,两种分型方法的一致率为93 33 % ( 2 8/ 30 )。表明山东省部分地区献血人群的HCV感染率较高 ;献血浆是该献血人群HCV感染的危险因素 ;To investigate the distribution of HCV infection and subtypes among blood donors in some areas of Shandong Province. Method Serum samples were collected from 424 paid blood donors. Anti-HCV was detected by enzyme-linked immunosorbent assay (ELISA).Serotypes of anti-HCV positive sera were identified by ELISA and genotypes were determined by RT-PCR.Results The HCV prevalence of 424 blood donors was 21 .70% (92/424). It was significantly higher in the donors donating blood and plasma (45. 14%) than in the donors with only blood donation (6.94%). Of 92 anti-HCV positive sera detected, 32 (34.78%) were typed by ELISA. 30 (93.75%) were serotype I, and 2 (6.25%) serotype Ⅱ. Of these 32 sera, 30 were determined by RT-PCR genotype method. HCV genotypes Ⅱ,Ⅲ and Ⅱ/Ⅲ were 26 (87.67%),3(10%), and Ⅰ(3.33%), respectively. The coincidence rate of the two methods was 93.33% (28/30).Conclusion The prevalence of HCV among blood donors in some areas of Shandong Province was relatively high. Plasma-donation was a risk factor of HCV infection in blood donors.The prevalent subtypes of HCV were serotype I and genotype Ⅱ.

关 键 词：丙型肝炎 献血员 血清学分型 基因分型 

分 类 号：R512.63[医药卫生—内科学]