日本血吸虫未成熟虫卵可溶性抗原54kD等抗原保护力的鉴定  

Identification of protective immunity elicited soluble by SIEA 54kD and other antigens

作　　者：蒋明森[1] 杨明义[1] 余祖江[1] 董惠芬[1] 陈晓蓓[1] 

机构地区：[1]湖北医科大学寄生虫学教研室,武汉430071

出　　处：《基础医学与临床》2000年第3期52-55,共4页Basic and Clinical Medicine

基　　金：湖北省教育委员会资助!970375

摘　　要：用SDS－PAGE制得的日本血吸虫未成熟虫卵可溶性抗原SIEA54kD抗原免疫小鼠，用尾蚴攻击感染后，其肝内的总虫卵数和成熟卵的比例与对照组小鼠比较有显著的减少。SIEA54kD抗原分子诱导的免疫有很强抗雌虫生殖和抗胚胎发育效果，因此可望作为日本血吸虫抗病疫苗候选分子。SIEA 67kD、 54kD、 40kD purified by SDS-PAGE or SIEA-u (soluble immature egg antigen in urea),were injected into mice for four times. 42 days after infection with cercariae of Schistosoma. Japonicum, the micewere killed and total livers were collected. The result showed that the antigen 54kD elicited two immune effects:antiegg maturation and anti-fecundity.The total egg number in liver reduced 49.6% and the proportion of mature eggdecreased 27.7% (P<0.05 ), But the other three antinens had no above effects. Therefore, 54kD is supposed to bethe hopeful candidate molecule of anti-egg maturation an anti-fecundity vaccine-

关 键 词：日本血吸虫 虫卵 免疫 SIEA-54kD 疫苗 

分 类 号：R392[医药卫生—免疫学]