野生型p53对热休克蛋白90β基因表达的调控作用  

Regulatory role of wild-type p53 on the expression of human hsp90β gene

作　　者：张业[1] 崔玉坤[1] 吴宁华[1] 沈珝琲[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2000年第3期76-79,共4页Basic and Clinical Medicine

基　　金：自然科学基金!39770619;9930050;863课题!102-11-02-05

摘　　要：为了阐明野生型p53分热休克蛋白90β（hsp90β）基因表达调控中的作用，我们利用野生型p53的表达质粒转染Jurkat细胞，发现野生型p53可以剂量依赖方式抑制hsp90β基因的启动子活性，并且证实hsp90β基因的表达在mRNA和蛋白水平均明显减少。Hsp90 acts as a molecular chaperone both constitutively and in cell stress response. Mammollalian Hsp90is encoded by two individual genes, the hsp90α and β isoforms. The two genes are differentially regulated. It hasbeen known that Hsp90 binds to p53 and participates in the regulation of p53 activity. Our results show that wild-typep53 inhibits the expression of hsp90β gene at both mRNA and protein levels in a dose dependent manner. Regulatorymechanisms of p53 on hsp90β gene had been studied and discussed.

关 键 词：HSP90Β基因 基因表达调控 P53 热休克蛋白 

分 类 号：Q51[生物学—生物化学]